Hengyang Medical School, Institute of Pharmacy and Pharmacology, The Affiliated Nanhua Hospital, Department of Hepatobiliary Surgery, University of South China, Hengyang 421000, Hunan, China.
Department of Clinical Laboratory, The Second Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang 421000, Hunan, China.
Nano Lett. 2024 Jul 10;24(27):8311-8319. doi: 10.1021/acs.nanolett.4c01568. Epub 2024 Jun 27.
Developing ultrasensitive lateral flow immunoassays (LFIAs) has garnered significant attention in the field of point-of-care testing. In this study, a trimetallic dendritic nanozyme (Pd@Pt-Ru) was synthesized through Ru deposition on a Pd@Pt core and utilized to enhancing the sensitivity of LFIAs. Pd@Pt-Ru exhibited a value of 5.23 mM for detecting HO, which indicates an HO affinity comparable with that of horseradish peroxidase. The Ru surface layer reduces the activation energy barrier, which increases the maximum reaction rate. As a proof of concept, the proposed Pd@Pt-Ru nanozyme was incorporated into LFIAs (A-Pd@Pt-Ru-LFIAs) for detecting human chorionic gonadotropin (hCG). Compared with conventional gold nanoparticle (AuNP)-LFIAs, A-Pd@Pt-Ru-LFIAs demonstrated 250-fold increased sensitivity, thereby enabling a visible detection limit as low as 0.1 IU/L. True positive and negative rates both reached 100%, which renders the proposed Pd@Pt-Ru nanozyme suitable for detecting hCG in clinical samples.
制备超灵敏侧向流动免疫分析（LFIAs）在即时检测领域受到了广泛关注。本研究通过在 Pd@Pt 核上沉积 Ru 合成了一种三金属树枝状纳米酶（Pd@Pt-Ru），并将其用于提高 LFIAs 的灵敏度。Pd@Pt-Ru 对 HO 的检测 值为 5.23mM，表明其与辣根过氧化物酶的 HO 亲和力相当。Ru 表面层降低了活化能垒，从而提高了最大反应速率。作为概念验证，将所提出的 Pd@Pt-Ru 纳米酶纳入 LFIAs（A-Pd@Pt-Ru-LFIAs）中用于检测人绒毛膜促性腺激素（hCG）。与传统的金纳米粒子（AuNP）-LFIAs 相比，A-Pd@Pt-Ru-LFIAs 的灵敏度提高了 250 倍，从而实现了低至 0.1IU/L 的可见检测限。阳性和阴性的真确率均达到 100%，表明所提出的 Pd@Pt-Ru 纳米酶适用于检测临床样本中的 hCG。
