Murray M K, Verhage H G
Biol Reprod. 1985 Jun;32(5):1229-35. doi: 10.1095/biolreprod32.5.1229.
An estrogen-dependent polypeptide (CUPED), which was purified from uterine flushings of estrogen-treated cats, was localized in endometrial epithelial cells of cats using the peroxidase-antiperoxidase immunocytochemical staining procedure. Epithelial cells from animals treated with estradiol for 4, 7, or 14 days and estrogen-primed animals treated with progesterone for 2 days showed positive immunostaining. Staining was absent in untreated ovariectomized animals and in estrogen-primed animals treated with progesterone for 4 days. Specific cytoplasmic staining was confined to apical secretory granules in nonciliated cells of deep uterine glands. Staining was also commonly observed in the lumen of deep glands. Immunostaining was absent in the cells of the surface epithelium, stroma, and myometrium. In addition, other organs such as the oviduct, kidney, liver, pancreas, and lung showed no evidence of specific immunocytochemical staining. Therefore, the estrogen-dependent polypeptide obtained from uterine flushings of estrogen-treated ovariectomized cats is a uterine-specific secretory product that is packaged in apical cytoplasmic granules of uterine epithelial gland cells before being released into the uterine lumen.
一种从经雌激素处理的猫的子宫冲洗液中纯化出来的雌激素依赖性多肽（CUPED），采用过氧化物酶-抗过氧化物酶免疫细胞化学染色法定位在猫的子宫内膜上皮细胞中。用雌二醇处理4天、7天或14天的动物以及用孕酮处理2天的雌激素预处理动物的上皮细胞呈现阳性免疫染色。未处理的去卵巢动物以及用孕酮处理4天的雌激素预处理动物未出现染色。特异性细胞质染色局限于深部子宫腺非纤毛细胞的顶端分泌颗粒。在深部腺管腔中也常见染色。表面上皮、基质和肌层细胞未出现免疫染色。此外，诸如输卵管、肾脏、肝脏、胰腺和肺等其他器官未显示特异性免疫细胞化学染色的迹象。因此，从经雌激素处理的去卵巢猫的子宫冲洗液中获得的雌激素依赖性多肽是一种子宫特异性分泌产物，在释放到子宫腔之前被包装在子宫上皮腺细胞的顶端细胞质颗粒中。
