Scalzo C M, Li W G, Mavrogianis P A, Verhage H G
Department of Anatomy and Cell Biology, University of Illinois College of Medicine, Chicago 60680.
J Reprod Immunol. 1990 Jun;17(3):265-77. doi: 10.1016/0165-0378(90)90008-t.
In the cat, a high molecular weight (greater than 320 kDa) estrogen (E2)-dependent protein (CUPED) and a group of low molecular weight (28 kDa, 36 kDa, 41 kDa) progesterone (P)-dependent proteins (PDP) are synthesized by the epithelial cells of the deep uterine glands and secreted into the lumen. The present study was designed to investigate the possibility that other species synthesize proteins similar to CUPED and PDP in response to steroids. Uterine flushings and media from cultured endometrial explants obtained from E2 or P dominated animals were analyzed on Western blots for the presence of proteins which cross-react with antibodies raised against CUPED and PDP. CUPED antibody immunoreacted with a protein band at approximately the same molecular weight as CUPED in the media and flushings from E2-treated ferret, dog and baboon, but not from rat, mouse or hamster. In the cat, ferret and dog, specific immunocytochemical staining was observed in the apical portion of the secretory cells. In the baboon, staining was observed in both the basal and apical areas of the epithelial cells. The presence of CUPED in the uterine lumen during the E2-dominated phase of the reproductive cycle in several species suggests that CUPED may play a general role in providing the necessary biochemical milieu for reproductive success. The PDP antibody did not cross-react with proteins in the media of species other than cat, and therefore may be unique to the cat.
在猫中，高分子量（大于320 kDa）的雌激素（E2）依赖性蛋白（CUPED）和一组低分子量（28 kDa、36 kDa、41 kDa）的孕激素（P）依赖性蛋白（PDP）由子宫深部腺体的上皮细胞合成并分泌到腺腔内。本研究旨在探究其他物种是否会响应类固醇合成与CUPED和PDP类似的蛋白质。对从E2或P占主导地位的动物获得的子宫冲洗液和培养的子宫内膜外植体培养基进行蛋白质免疫印迹分析，以检测是否存在与针对CUPED和PDP产生的抗体发生交叉反应的蛋白质。CUPED抗体与来自经E2处理的雪貂、狗和狒狒的培养基及冲洗液中分子量与CUPED大致相同的一条蛋白带发生免疫反应，但与大鼠、小鼠或仓鼠的没有反应。在猫、雪貂和狗中，在分泌细胞的顶端部分观察到特异性免疫细胞化学染色。在狒狒中，在上皮细胞的基底和顶端区域均观察到染色。在几种物种的生殖周期中E2占主导地位的阶段，子宫腔内存在CUPED，这表明CUPED可能在为生殖成功提供必要的生化环境方面发挥普遍作用。PDP抗体与猫以外物种的培养基中的蛋白质没有交叉反应，因此可能是猫所特有的。
