Murray M K, Verhage H G, Jaffe R C
Biol Reprod. 1986 Oct;35(3):531-6. doi: 10.1095/biolreprod35.3.531.
We previously reported the purification of an estrogen-dependent cat uterine protein (CUPED) and the preparation of a specific anti-CUPED serum in rabbits. Here, we describe a specific radioimmunoassay for CUPED using the purified CUPED and anti-CUPED serum that was utilized to quantify CUPED in daily uterine flushings obtained from steroid-treated ovariectomized cats. The radioimmunoassay was sufficiently sensitive to measure 0.1-100 ng CUPED. CUPED levels were low in untreated ovariectomized cats, increased within one day after the onset of treatment with estradiol, and remained elevated as long as estradiol was unopposed by progesterone. The levels of CUPED decreased when progesterone was added to the treatment regimen either 7, 14, or 28 days after the initiation of estradiol treatment. The data indicate that the presence of CUPED in the uterine flushings is dependent on the presence of estradiol and the absence of progesterone, that CUPED appears in the uterine lumen within one day after the onset of treatment with estradiol, and that the levels of CUPED are sharply reduced within one day of administration of progesterone and become nondetectable after three days.
我们之前报道了雌激素依赖性猫子宫蛋白（CUPED）的纯化以及在兔体内制备特异性抗CUPED血清的过程。在此，我们描述了一种使用纯化的CUPED和抗CUPED血清对CUPED进行的特异性放射免疫测定法，该方法用于定量从接受类固醇处理的去卵巢猫每日子宫冲洗液中获取的CUPED。该放射免疫测定法灵敏度足够高，能够检测0.1 - 100 ng的CUPED。在未处理的去卵巢猫中，CUPED水平较低，在用雌二醇治疗开始后的一天内升高，并且只要雌二醇未受到孕酮的拮抗，其水平就会一直保持升高。当在雌二醇治疗开始后的第7天、14天或28天向治疗方案中添加孕酮时，CUPED水平会下降。数据表明，子宫冲洗液中CUPED的存在取决于雌二醇的存在以及孕酮的缺失，CUPED在雌二醇治疗开始后的一天内出现在子宫腔中，并且在给予孕酮后的一天内CUPED水平会急剧下降，三天后则无法检测到。
