ARKRAY, Inc., Research and Development Division, Yousuien-nai, 59 Gansuin-cho, Kamigyo-ku, Kyoto 602-0008, Japan.
Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan.
J AOAC Int. 2024 Nov 1;107(6):943-952. doi: 10.1093/jaoacint/qsae053.
Mercury intake is caused by eating seafood, such as tuna and other predatory fish species. To reduce the health risks of mercury intake, it is necessary to continuously measure and monitor mercury concentrations at fish farms and markets. We have developed a compact system that can detect multiple heavy metals by liquid asymmetric-electrode plasma optical emission spectroscopy (LAEP-OES).
The validity of the LAEP-OES method for total mercury levels was evaluated using standard solutions, certified substances, and specimens of bluefin tuna and other fish species.
All specimens were dissolved in 4 M lithium hydroxide solution and then dispensed into a sample reservoir well of the single-use measurement reagent pack. Total mercury levels were automatically measured within 15 min of placement into the dedicated equipment. A total of 102 fish specimens, classified into 10 fish species, were evaluated using the new method and the results were compared to those obtained from validated analytical methods.
LOD (0.02 mg/kg), LOQ (0.07 mg/kg), repeatability (4.0%), intermediate precision (9.8%), and trueness (recoveries 107%) of the proposed method were within satisfactory limits for total mercury levels in fish. Additionally, when using various fish species, the method had a strong positive correlation with the results of cold-vapor atomic absorption spectrometry (CV-AAS, the official method) with Spearman rs = 0.984.
The LAEP-OES method can be used for measuring total mercury levels in bluefin tuna. Total mercury measurement using this new method has the potential to be applied to other fish species.
Total mercury levels in fish were measured using our unique analysis system. Pacific bluefin tuna, southern bluefin tuna, and Atlantic bluefin tuna distributed in the Japanese market were analyzed for total mercury in their wild and farmed fish varieties.
汞的摄入是由于食用了金枪鱼和其他掠食性鱼类等海鲜。为了降低汞摄入的健康风险，有必要不断测量和监测养殖场和市场上的汞浓度。我们开发了一种紧凑的系统，可以通过液体不对称电极等离子体光学发射光谱法（LAEP-OES）检测多种重金属。
使用标准溶液、认证物质以及蓝鳍金枪鱼和其他鱼类的标本，评估 LAEP-OES 方法测定总汞水平的有效性。
所有标本均溶解在 4 M 氢氧化锂溶液中，然后分注到一次性测量试剂包的样品储存井中。标本放入专用设备后 15 分钟内自动测量总汞水平。使用新方法评估了 102 个鱼类标本，分为 10 个鱼类物种，并将结果与经过验证的分析方法进行了比较。
所提出方法测定鱼类总汞的检出限（0.02mg/kg）、定量限（0.07mg/kg）、重复性（4.0%）、中间精密度（9.8%）和准确度（回收率 107%）均在鱼类总汞水平的可接受范围内。此外，当使用各种鱼类物种时，该方法与冷蒸气原子吸收光谱法（CV-AAS，官方方法）的结果具有很强的正相关性（Spearman rs=0.984）。
LAEP-OES 方法可用于测定蓝鳍金枪鱼的总汞水平。使用这种新方法测定总汞有可能应用于其他鱼类物种。
使用我们独特的分析系统测定鱼类中的总汞水平。分析了在日本市场上分布的野生和养殖的太平洋蓝鳍金枪鱼、南方蓝鳍金枪鱼和大西洋蓝鳍金枪鱼的总汞含量。
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