Boeheim K, Speak J A, Frei E, Bernal S D
Int J Cancer. 1985 Aug 15;36(2):137-42. doi: 10.1002/ijc.2910360203.
We produced murine monoclonal antibodies (MAbs) directed against the surface membrane of squamous-cell carcinoma of the head and neck (SCCHN). One antibody, SQM1, was determined by immunofluorescence and radioimmunoassay to be reactive with 13/13 SCCHN cell lines derived from different sites of the head and neck area. No binding reaction was observed with normal fibroblasts, red blood cells, nucleated bone-marrow cells or epithelial cells from normal oral mucosa. SQM1 reactivity was observed with primary cultures of normal epidermal and bronchial epithelial cells. Significant reactivity was found with 2/4 cell lines derived from small-cell carcinoma of the lung but little or no reactivity was found with other lung cancer cell lines. Various cell lines derived from other cancers including breast, colon, and ovarian carcinomas, melanoma, neuroblastoma and leukemia were generally unreactive. Seventeen out of 18 fresh frozen specimens of SCCHN were strongly reactive with SQM1 antibody. However, autopsy specimens from the heart, liver, kidney, spleen, colon, subcutaneous fat and skin connective tissue were unreactive. SQM1 antibody may be useful in biological and clinical studies of the head and neck region.
我们制备了针对头颈部鳞状细胞癌（SCCHN）表面膜的鼠单克隆抗体（MAb）。通过免疫荧光和放射免疫测定法确定，一种抗体SQM1可与源自头颈部不同部位的13/13种SCCHN细胞系发生反应。未观察到与正常成纤维细胞、红细胞、有核骨髓细胞或正常口腔黏膜上皮细胞发生结合反应。在正常表皮和支气管上皮细胞的原代培养物中观察到了SQM1反应性。在源自肺小细胞癌的2/4种细胞系中发现了显著反应性，但在其他肺癌细胞系中发现的反应性很小或没有反应性。源自其他癌症（包括乳腺癌、结肠癌和卵巢癌、黑色素瘤、神经母细胞瘤和白血病）的各种细胞系通常无反应性。18份SCCHN新鲜冷冻标本中有17份与SQM1抗体发生强烈反应。然而，来自心脏、肝脏、肾脏、脾脏、结肠、皮下脂肪和皮肤结缔组织的尸检标本无反应性。SQM1抗体可能对头颈部区域的生物学和临床研究有用。
