Yuan C C, Tsai L C, Hsu S C, Ng H T, Tsai S J, Chen H M, Hung M W, Ho C K, Ho D M, Gill T J
Department of Obstetrics and Gynecology, Veterans General Hospital, Taipei, Taiwan, ROC.
Br J Cancer. 1992 Feb;65(2):201-7. doi: 10.1038/bjc.1992.42.
An IgG1 monoclonal antibody (MAb Cx-99) has been established which recognises a surface antigen on epithelial cells, but not on fibroblastic or hematopoietic cells. Immunohistochemical studies showed that this antigen was present in all 37 squamous cell carcinomas (SCC) including 33 cervical SCC, and 30 of the 32 adenocarcinomas examined; most of the 33 cervical SCC were stained extensively. It was also detected in the culture medium of cervical cancer cell lines. In the normal cervix, this antigen was restricted to the undifferentiated basal cells. This observation suggests that the widespread expression of the antigen was triggered by oncogenesis. The MAb Cx-99 recognised an epitope on an asialyted glycoprotein which has an apparent molecular weight of 37 kilodaltons (kD) (and 2 minor proteins at 18 and 27 kD) and an isoelectric point (pI) of 5.3. It may have potential for studies on differentiation and oncogenesis and for diagnostic applications.
已制备出一种IgG1单克隆抗体（MAb Cx - 99），它能识别上皮细胞表面的一种抗原，但不能识别成纤维细胞或造血细胞表面的该抗原。免疫组织化学研究表明，这种抗原存在于所有37例鳞状细胞癌（SCC）中，包括33例宫颈鳞状细胞癌，以及所检测的32例腺癌中的30例；33例宫颈鳞状细胞癌中的大多数都有广泛染色。在宫颈癌细胞系的培养基中也检测到了这种抗原。在正常宫颈中，这种抗原仅限于未分化的基底细胞。这一观察结果表明，该抗原的广泛表达是由肿瘤发生引发的。MAb Cx - 99识别一种去唾液酸糖蛋白上的一个表位，该糖蛋白的表观分子量为37千道尔顿（kD）（还有两个次要蛋白，分子量分别为18 kD和27 kD），等电点（pI）为5.3。它在分化和肿瘤发生研究以及诊断应用方面可能具有潜力。
