Inoue M, Nakanishi K, Sasagawa T, Tanizawa O, Inoue H, Hakura A
Department of Obstetrics and Gynecology, Osaka University Medical School.
Jpn J Cancer Res. 1990 Feb;81(2):176-82. doi: 10.1111/j.1349-7006.1990.tb02545.x.
The monoclonal antibody, INS-2, was raised against rat fibroblasts transformed by open reading frames E6 and E7 of human papillomavirus (HPV) DNA. In immunoperoxidase testing of frozen sections, the INS-2 antibody was reactive with all squamous cell carcinomas of the uterine cervix and esophagus tested. In contrast, no antibody binding was detected with adenocarcinomas of various origins. Similarly, normal tissues, lymphoid cells and erythrocytes from multiple donors were negative, except that binding localized at basal cells in normal squamous epithelium was observed. Interestingly, strong staining was observed in dysplastic cells of cervical intraepithelial neoplasia and at the growing edge of squamous cell carcinomas. The antigen for the INS-2 antibody is a non-sialyl glycoprotein with Mw. 40,000 and appears to be a squamous cell-specific cell differentiation marker, although it is not related to HPV-DNA-derived protein.
单克隆抗体INS-2是针对由人乳头瘤病毒（HPV）DNA的开放阅读框E6和E7转化的大鼠成纤维细胞产生的。在冷冻切片的免疫过氧化物酶检测中，INS-2抗体与所有检测的子宫颈和食管鳞状细胞癌发生反应。相比之下，未检测到与各种来源腺癌的抗体结合。同样，来自多个供体的正常组织、淋巴细胞和红细胞均为阴性，不过在正常鳞状上皮的基底细胞中观察到有结合定位。有趣的是，在宫颈上皮内瘤变的发育异常细胞以及鳞状细胞癌的生长边缘观察到强染色。INS-2抗体的抗原是一种分子量为40,000的非唾液酸化糖蛋白，尽管它与HPV-DNA衍生蛋白无关，但似乎是一种鳞状细胞特异性细胞分化标志物。
