School of Agriculture and Biotechnology, Shenzhen Campus of Sun Yat-sen University, Shenzhen 518107, China.
Dell Pediatric Research Institute, Depts of Pediatrics, of Chemistry, and of Nutrition, University of Texas at Austin, 1400 Barbara Jordan Blvd, Austin, Texas 78723, United States.
J Agric Food Chem. 2024 Jul 10;72(27):15311-15320. doi: 10.1021/acs.jafc.4c03546. Epub 2024 Jun 29.
Omega-3 long-chain polyunsaturated fatty acids (LCPUFA) play critical roles in human development and health. Their intake is often effectively estimated solely based on seafood consumption, though the high intake of terrestrial animal-based foods with minor amounts of LCPUFA may be significant. Covalent adduct chemical ionization (CACI) tandem mass spectrometry is one approach for structural and quantitative analysis of minor unsaturated fatty acids (FA), for which standards are unavailable. Here, CACI-MS and MS/MS are used to identify and quantify minor omega-3 LCPUFA of terrestrial animal foods based on the application of measured response factors (RFs) to various FA. American mean intakes of pork, beef, chicken, and eggs contribute 20, 27, 45, and 71 mg/day of docosahexaenoic acid (DHA), respectively. The estimated intake of omega-3 DHA, eicosapentaenoic acid, and docosapentaenoic acid from nonseafood sources is significant, at 164, 103, and 330 mg/day, greater than most existing estimates of omega-3 LCPUFA intake.
ω-3 长链多不饱和脂肪酸（LCPUFA）在人类发育和健康中发挥着关键作用。它们的摄入量通常仅通过海鲜摄入量来有效估计，尽管大量摄入陆地动物源性食品，其中 LCPUFA 含量很少，可能也很重要。共价加合物化学电离（CACI）串联质谱法是一种用于分析微量不饱和脂肪酸（FA）结构和定量的方法，因为这些 FA 没有标准品。在这里，基于对各种 FA 应用测量的响应因子（RF），CACI-MS 和 MS/MS 用于鉴定和定量陆地动物食品中的微量 ω-3 LCPUFA。美国猪肉、牛肉、鸡肉和鸡蛋的平均摄入量分别贡献了 20、27、45 和 71 毫克/天的二十二碳六烯酸（DHA）。从非海鲜来源估计的 ω-3 DHA、二十碳五烯酸和二十二碳五烯酸的摄入量分别为 164、103 和 330 毫克/天，明显高于大多数现有的 ω-3 LCPUFA 摄入量估计值。
