Skovgaard N, Christensen S G, Gulistani A W
J Hyg (Lond). 1985 Aug;95(1):69-75. doi: 10.1017/s002217240006229x.
Caecal samples from 350 Danish bacon pigs were investigated for salmonella using three methods of isolation. (1) Direct inoculation of 1 g of faeces into 10 ml of Muller-Kaufmann medium (MK medium) with addition of 0.3% Teepol 610 and subculture on Brilliant Green lactose sucrose phenol-red agar (BLSF agar) with 0.3% Teepol 610. (2) Pre-enrichment of 5 g of faeces into buffered peptone water with addition of 1% Teepol 610 followed by enrichment of 1 ml in 10 ml MK medium with 1% Teepol 610 and subculture on BLSF agar with 0.3% Teepol. (3) Incubation of 0.1 ml of the pre-enrichment (2) into 10 ml Rappaport-Vassiliadis medium (RV 10 medium) incubated at 43 degrees C, subculture on BLSF agar. The MK media with and without pre-enrichment yielded higher findings than the RV 10 media. In total, 28 (8%) of the pigs were found positive, representing 11 (7.4%) of a total of 142 herds investigated. Lymph glands were collected at a later date from six of the positive herds. Five of the herds were found positive. The number of salmonellas in the glands was low, probably less than ten per gram.
使用三种分离方法对350头丹麦培根猪的盲肠样本进行了沙门氏菌检测。（1）将1克粪便直接接种到10毫升添加了0.3%吐温610的Muller-Kaufmann培养基（MK培养基）中，并在添加了0.3%吐温610的煌绿乳糖蔗糖酚红琼脂（BLSF琼脂）上进行传代培养。（2）将5克粪便在添加了1%吐温610的缓冲蛋白胨水中进行预富集，然后将1毫升富集液接种到10毫升添加了1%吐温610的MK培养基中，并在添加了0.3%吐温的BLSF琼脂上进行传代培养。（3）将0.1毫升预富集液（2）接种到10毫升Rappaport-Vassiliadis培养基（RV 10培养基）中，在43摄氏度下培养，然后在BLSF琼脂上进行传代培养。经过预富集和未经过预富集的MK培养基检测结果均高于RV 10培养基。总共发现28头（8%）猪呈阳性，占所检测的142个猪群中11个（7.4%）。随后从6个阳性猪群中采集了淋巴结。发现其中5个猪群呈阳性。淋巴结中的沙门氏菌数量较少，每克可能少于10个。
