基于Rappaport-Vassiliadis培养基的方法与使用含特波尔的Muller-Kauffmann培养基分离沙门氏菌属的方法的比较
Comparison of procedures based upon Rappaport-Vassiliadis medium with those using Muller-Kauffmann medium containing Teepol for the isolation of Salmonella sp.
作者信息
Vassiliadis P, Mavromati C, Trichopoulus D, Kalapothaki V, Papadakis J
出版信息
Epidemiol Infect. 1987 Aug;99(1):143-7. doi: 10.1017/s0950268800066954.
Abstract
A total of 308 samples of different types were examined for the presence of salmonellas by means of three different procedures. The first consisted of pre-enrichment in buffered peptone water followed by enrichment in Rappaport-Vassiliadis medium (P/RV). The second differed only in that 1% Teepol was added to the pre-enrichment medium (PT/RV). In the third, buffered peptone water with 1% Teepol was followed by enrichment in Muller-Kauffmann tetrathionate broth also containing 1% Teepol (PT/MKT). The first of these combinations (P/RV) proved superior to the others both in terms of isolation rates and in the appearance of suspicious colonies.
摘要
通过三种不同的方法对总共308份不同类型的样本进行了沙门氏菌检测。第一种方法是先在缓冲蛋白胨水中进行预富集，然后在Rappaport-Vassiliadis培养基（P/RV）中进行富集。第二种方法的唯一不同之处在于，在预富集培养基中添加了1%的吐温80（PT/RV）。第三种方法是先在含有1%吐温80的缓冲蛋白胨水中进行预富集，然后在同样含有1%吐温80的Muller-Kauffmann四硫磺酸盐肉汤（PT/MKT）中进行富集。就分离率和可疑菌落的出现情况而言，这些组合中的第一种（P/RV）被证明优于其他组合。
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