College of Life Sciences, Shaanxi Normal University, 710119 Xi'an, China.
Genome Biol Evol. 2024 Jul 3;16(7). doi: 10.1093/gbe/evae145.
Oecanthus is a genus of cricket known for its distinctive chirping and distributed across major zoogeographical regions worldwide. This study focuses on Oecanthus rufescens, and conducts a comprehensive examination of its genome through genome sequencing technologies and bioinformatic analysis. A high-quality chromosome-level genome of O. rufescens was successfully obtained, revealing significant features of its genome structure. The genome size is 877.9 Mb, comprising ten pseudo-chromosomes and 70 other sequences, with a GC content of 41.38% and an N50 value of 157,110,771 bp, indicating a high level of continuity. BUSCO assessment results demonstrate that the genome's integrity and quality are high (of which 96.8% are single-copy and 1.6% are duplicated). Comprehensive genome annotation was also performed, identifying approximately 310 Mb of repetitive sequences, accounting for 35.3% of the total genome sequence, and discovering 15,481 tRNA genes, 4,082 rRNA genes, and 1,212 other noncoding genes. Furthermore, 15,031 protein-coding genes were identified, with BUSCO assessment results showing that 98.4% (of which 96.3% are single-copy and 1.6% are duplicated) of the genes were annotated.
Oecanthus 是一种以其独特的鸣叫声而闻名的蟋蟀属，分布在全球各大动物地理区域。本研究聚焦于 Oecanthus rufescens，通过基因组测序技术和生物信息学分析对其基因组进行了全面研究。成功获得了高质量的 O. rufescens 染色体水平基因组，揭示了其基因组结构的显著特征。该基因组大小为 877.9 Mb，由十个假染色体和其他 70 个序列组成，GC 含量为 41.38%，N50 值为 157,110,771 bp，表明其具有高度的连续性。BUSCO 评估结果表明，该基因组具有较高的完整性和质量（其中单拷贝基因为 96.8%，重复基因为 1.6%）。还进行了全面的基因组注释，鉴定出约 310 Mb 的重复序列，占总基因组序列的 35.3%，发现了 15,481 个 tRNA 基因、4,082 个 rRNA 基因和 1,212 个其他非编码基因。此外，鉴定出 15,031 个蛋白质编码基因，BUSCO 评估结果显示，其中 98.4%（单拷贝基因为 96.3%，重复基因为 1.6%）的基因得到了注释。
