Zhang Xinyuan, Fan Ping, Liu Qingqing, Liu Xinghui, Bai Huai, Wu Yujie, Li Suiyan
School of Life Science and Engineering, Southwest Jiaotong University, Chengdu, Sichuan 610031, China.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2024 Jul 10;41(7):866-871. doi: 10.3760/cma.j.cn511374-20230515-00287.
To assess the association of -c.108C>T and c.192Q>R polymorphisms of paraoxonase 1 (PON1) gene with preeclampsia (PE) and the influence of genotypes on the metabolic and oxidative stress indexes among Chinese women.
This case-control study has included 334 patients with PE and 1337 healthy pregnant women. The -c.108C>T and c.192Q>R genotypes were determined by PCR and restriction fragment length polymorphism method. Metabolic and oxidative stress parameters were also analyzed.
No statistical difference in the genotypic and allelic frequencies for the -c.108C>T and c.192Q>R polymorphisms of the PON1 gene was found between the PE patients and the healthy controls (P > 0.05). Nevertheless, the 192Q-108T haplotype of these polymorphisms was associated with an increased risk of PE (P = 0.007). Total antioxidant capacity (TAC) and atherosderosis index were higher in patients with the -108TT genotype compared with those with a CT genotype (P < 0.05); whilst total oxidant status was lower in patients with a CT genotype compared with those with a CC genotype (P = 0.036). Malondialdehyde level was higher in patients with a 192RR genotype compared with those with a QQ genotype (P = 0.019). TAC level was higher in patients with a RR genotype compared with those with a QR genotype (P = 0.015).
The 192Q-108T haplotype of the PON1 gene is associated with the risk for PE. These polymorphisms may be associated with abnormal lipid metabolism and oxidative stress among Chinese PE patients.
评估对氧磷酶1（PON1）基因-c.108C>T和c.192Q>R多态性与子痫前期（PE）的相关性，以及这些基因型对中国女性代谢和氧化应激指标的影响。
本病例对照研究纳入了334例PE患者和1337例健康孕妇。采用聚合酶链反应（PCR）和限制性片段长度多态性方法确定-c.108C>T和c.192Q>R基因型。同时分析代谢和氧化应激参数。
PE患者与健康对照之间，PON1基因-c.108C>T和c.192Q>R多态性的基因型和等位基因频率无统计学差异（P>0.05）。然而，这些多态性的192Q-108T单倍型与PE风险增加相关（P=0.007）。-108TT基因型患者的总抗氧化能力（TAC）和动脉粥样硬化指数高于CT基因型患者（P<0.05）；而CT基因型患者的总氧化剂状态低于CC基因型患者（P=0.036）。192RR基因型患者的丙二醛水平高于QQ基因型患者（P=0.019）。RR基因型患者的TAC水平高于QR基因型患者（P=0.015）。
PON1基因的192Q-108T单倍型与PE风险相关。这些多态性可能与中国PE患者的脂质代谢异常和氧化应激有关。
