Silage D A, Gil J
J Microsc. 1985 May;138(Pt 2):221-7. doi: 10.1111/j.1365-2818.1985.tb02615.x.
Segmentation of large areas of light microscopic slides into N by N fields, and each of these fields into M digital image tiles, allows the scanning, storage and digital processing of large images. Any of the original N2 fields or composites of M adjacent tiles can be recalled to the video display for analysis. Developed procedures for use on a microscope equipped with a precision scanning stage allow registration of the image coordinates (X-Y) for any original or composite field and the alignment of one of these fields along the depth (Z) axis by means of external, machined fiducial marks in serial sections. To facilitate work whenever unavoidable, we have incorporated methods for digital image panning and zooming (changes of magnification) and discuss their use and implications.
将大面积光学显微镜载玻片分割成N×N个视野，每个视野再分割成M个数字图像块，这样就能对大图像进行扫描、存储和数字处理。任何一个原始的N²个视野或M个相邻图像块的组合都可以调回到视频显示器进行分析。在配备精密扫描台的显微镜上使用的已开发程序，允许对任何原始或组合视野的图像坐标（X-Y）进行配准，并通过连续切片中外部加工的基准标记，使这些视野之一沿深度（Z）轴对齐。为了在不可避免的情况下方便工作，我们纳入了数字图像平移和缩放（放大倍数变化）的方法，并讨论了它们的用途和影响。
