Gonkowski Sławomir, Martín Julia, Rychlik Andrzej, Aparicio Irene, Santos Juan Luis, Alonso Esteban, Makowska Krystyna
Department of Clinical Physiology, Olsztyn, Poland.
Departamento de Química Analítica, Escuela Politécnica Superior, Universidad de Sevilla, E-41011 Sevilla, Spain.
J Vet Res. 2024 Apr 11;68(2):303-312. doi: 10.2478/jvetres-2024-0022. eCollection 2024 Jun.
Benzophenones (BPs) are used in various branches of industry as ultraviolet radiation filters, but they pollute the natural environment, penetrate living organisms, and disrupt endocrine balance. Knowledge of the exposure of domestic animals to these substances is extremely scant. The aim of the study was to investigate long-term exposure of companion dogs to BPs and relate this to environmental factors.
Hair samples taken from 50 dogs and 50 bitches from under 2 to over 10 years old were analysed for BP content with liquid chromatography-tandem mass spectrometry.
The results revealed that dogs are most often exposed to 2-hydroxy-4-methoxybenzophenone (BP-3) and 4-dihydroxybenzophenone (BP-1). Concentration levels of BP-3 above the method quantification limit (MQL) were noted in 100% of the samples and fluctuated from 4.75 ng/g to 1,765 ng/g. In turn, concentration levels of BP-1 above the MQL were noted in 37% of the samples and ranged from <0.50 ng/g to 666 ng/g. Various factors (such as the use of hygiene and care products and the dog's diet) were found to affect BP concentration levels. Higher levels of BP-3 were observed in castrated/spayed animals and in animals that required veterinary intervention more often.
The results obtained show that the analysis of hair samples may be a useful matrix for biomonitoring BPs in dogs, and that these substances may be toxic to them.
二苯甲酮（BPs）在工业的各个分支中用作紫外线辐射过滤器，但它们会污染自然环境，渗透到生物体内，并破坏内分泌平衡。关于家畜接触这些物质的情况，人们知之甚少。本研究的目的是调查宠物狗长期接触二苯甲酮的情况，并将其与环境因素联系起来。
采集了50只年龄在2岁以下至10岁以上的公狗和50只母狗的毛发样本，采用液相色谱-串联质谱法分析其中二苯甲酮的含量。
结果显示，狗最常接触的是2-羟基-4-甲氧基二苯甲酮（BP-3）和4-二羟基二苯甲酮（BP-1）。100%的样本中BP-3的浓度水平高于方法定量限（MQL），波动范围为4.75纳克/克至1765纳克/克。而37%的样本中BP-1的浓度水平高于MQL，范围为<0.50纳克/克至666纳克/克。研究发现，各种因素（如使用卫生和护理产品以及狗的饮食）会影响二苯甲酮的浓度水平。在去势/绝育的动物以及更经常需要兽医干预的动物中，观察到较高水平的BP-3。
所得结果表明，毛发样本分析可能是监测狗中二苯甲酮的有用基质，并且这些物质可能对它们有毒。
