硫角菌属一新种，从表层海水中分离得到的一种新型 α-变形菌。
Thioclava litoralis sp. nov., a novel species of alphaproteobacterium, isolated from surface seawater.
机构信息
Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy culture, Key Laboratory of Control for Disease of Aquatic Animals of Guangdong Higher Education Institute, College of Fishery, Guangdong Ocean University, Zhanjiang, China.
Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment, Shenzhen Public Service Platform for Evaluation of Marine Economic Animal Seedings, Shenzhen Institute of Guangdong Ocean University, Shenzhen, China.
出版信息
Arch Microbiol. 2024 Jun 29;206(7):333. doi: 10.1007/s00203-024-04057-6.
A Gram-negative, aerobic, rod-shaped, non-motile bacterium, designated as FTW29, was isolated from surface seawater sampled in Futian district, Shenzhen, China. Growth of strain FTW29 was observed at 15-42 ℃ (optimum, 28-30 ℃), pH 4.0-9.0 (optimum, pH 5.5-7.5) and in the presence of 0.5-10% NaCl (optimum, 3.0% NaCl). Strain FTW29 showed 95.0-96.8% 16 S rRNA gene sequence similarity to various type strains of the genera Thioclava, Sinirhodobacter, Rhodobacter, Haematobacter and Frigidibacter of the family Paracoccaceae, and its most closely related strains were Thioclava pacifica DSM 10,166 (96.8%) and Thioclava marina 11.10-0-13 (96.7%). The phylogenomic tree constructed on the bac120 gene set showed that strain FTW29 formed a clade with the genus Thioclava, with a bootstrap value of 100%. The evolutionary distance values between FTW29 and type strains of the genus Thioclava were 0.17-0.19, which are below the recommended standard (0.21-0.23) for defining a novel genus in the family Paracoccaceae. In strain FTW29, the major fatty acids identified were summed feature 8 (Cω7c) and C and the predominant respiratory quinones were ubiquinone-10 and ubiquinone-9. The composition of polar lipids in strain FTW29 included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified phospholipid, an unidentified aminolipid, two unidentified glycolipids and an unidentified lipid. The genome of strain FTW29 comprised one circle chromosome and six plasmids, with a G + C content of 61.4%. The average nucleotide identity, average amino acid identity, and digital DNA-DNA hybridization values between strain FTW29 and seven type strains of the genus Thioclava were 76.6-78.4%, 53.2-56.4% and 19.3-20.4%, respectively. Altogether, the phenotypic, phylogenetic and chemotaxonomic evidence illustrated in this study suggested that strain FTW29 represents a novel species of the genus Thioclava, with the proposed name Thioclava litoralis sp. nov. The type strain is FTW29 (= KCTC 82,841 = MCCC 1K08523).
一株革兰氏阴性、需氧、杆状、非运动细菌，被命名为 FTW29，从中国深圳福田区采集的表层海水中分离得到。菌株 FTW29 的生长温度为 15-42℃（最佳温度为 28-30℃），pH 值为 4.0-9.0（最佳 pH 值为 5.5-7.5），能在 0.5-10%NaCl（最佳为 3.0%NaCl）的环境中生长。菌株 FTW29 的 16S rRNA 基因序列与 Paracoccaceae 家族的 Thioclava、Sinirhodobacter、Rhodobacter、Haematobacter 和 Frigidibacter 属的各种模式菌株的相似性为 95.0-96.8%，与其最密切相关的菌株为 Thioclava pacifica DSM 10,166（96.8%）和 Thioclava marina 11.10-0-13（96.7%）。基于 bac120 基因构建的系统发育树表明，菌株 FTW29 与 Thioclava 属形成了一个分支，其自举值为 100%。FTW29 与 Thioclava 属模式菌株之间的进化距离值为 0.17-0.19，低于 Paracoccaceae 家族中定义新属的推荐标准（0.21-0.23）。在菌株 FTW29 中，鉴定出的主要脂肪酸为 summed feature 8（Cω7c）和 C，主要呼吸醌为 ubiquinone-10 和 ubiquinone-9。菌株 FTW29 的极性脂包括双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、一种未鉴定的磷脂、一种未鉴定的胺脂、两种未鉴定的糖脂和一种未鉴定的脂。菌株 FTW29 的基因组由一个圆形染色体和六个质粒组成，G+C 含量为 61.4%。菌株 FTW29 与 Thioclava 属的 7 个模式菌株之间的平均核苷酸同一性、平均氨基酸同一性和数字 DNA-DNA 杂交值分别为 76.6-78.4%、53.2-56.4%和 19.3-20.4%。总之，本研究的表型、系统发育和化学分类学证据表明，菌株 FTW29 代表了 Thioclava 属的一个新种，命名为 Thioclava litoralis sp. nov. 其模式菌株为 FTW29（=KCTC 82841=MCCC 1K08523）。
Zotero 插件