Department of Biotechnology, Institute of Allied Health (Paramedical) Services, Education and Training, Uttarakhand Forest Hospital Trust, Rampur Road, Haldwani, 263139, Uttarakhand, India.
Appl Biochem Biotechnol. 2010 Mar;160(5):1356-61. doi: 10.1007/s12010-009-8577-0. Epub 2009 Mar 5.
The antibacterial activity of Aloe barbadensis was tested on clinically isolated bacterial pathogens i.e. Enterococcus bovis, Staphylococcus aureus, Escherichia coli, Proteus vulgaris, Proteus mirabilis, Pseudomonas aeruginosa, Morganella morganii, and Klebsiella pneumoniae causing infection in human being. Ethanolic and aqueous extracts were used for the antibacterial effect, which was measured by the appearance of zone of inhibition. Relatively higher MIC concentrations were obtained for gram negative bacteria E. coli and K. pneumoniae, with ethanol extract; however, no inhibitory effect was noted for aqueous extract. Ethanolic extract possesses great inhibitory activity for gram positive bacteria, E. bovis followed by S. aureus. Among gram negative bacteria, highest inhibitory effect was observed with P. aeruginosa, followed by M. morganii, P. mirabilis, and P. vulgaris, which was significant (p < 0.01) than E. coli and K. pneumoniae. Antimicrobial activity tests of crude extract of A. barbadensis were carried out to validate the use of traditional medicinal herbal and results of this study tend to give credence to the common use of A. barbadensis gel and leaf.
对库拉索芦荟进行了体外抗菌活性试验，分离出临床感染的病原菌，即牛链球菌、金黄色葡萄球菌、大肠杆菌、普通变形杆菌、奇异变形杆菌、绿脓假单胞菌、摩氏摩根菌和肺炎克雷伯菌。采用琼脂扩散法，以抑菌圈直径大小评价抑菌活性，分别用乙醇提取物和水提取物进行试验。对于革兰氏阴性菌大肠杆菌和肺炎克雷伯菌，乙醇提取物的 MIC 浓度相对较高；然而，水提取物对它们没有抑制作用。对于革兰氏阳性菌，乙醇提取物具有很强的抑制活性，依次为牛链球菌和金黄色葡萄球菌。在革兰氏阴性菌中，对绿脓假单胞菌的抑制作用最强，其次是摩氏摩根菌、奇异变形杆菌和普通变形杆菌，与大肠杆菌和肺炎克雷伯菌相比，差异有统计学意义（p<0.01）。对库拉索芦荟粗提取物进行了抗菌活性试验，以验证传统药用草药的使用，本研究结果为库拉索芦荟凝胶和叶的常规使用提供了依据。
