Pinon-Lataillade G, Maas J
Strahlentherapie. 1985 Jul;161(7):421-6.
Male Sprague Dawley rats were continuously irradiated at a dose-rate of either 5 or 7 cGy/day, up to a total dose of 900 cGy. Changes in spermatogenesis with irradiation and the recovery of the testis during 33 weeks after irradiation were studied. No clear dose-rate effect with testicular weight occurred. During the irradiation time, increased dose and dose-rate induced a decrease in A spermatogonia and preleptotene spermatocyte number. In our experimental conditions germ cell production did not plateau, as shown by the increasing number of tubular cross sections devoid of germ cells beyond 500 cGy. The recovery of seminiferous epithelium occurred essentially within nine weeks. It was not dose-rate dependent and was still incomplete after 33 weeks. This lack of recovery might be due to limited compensatory division ability of the stem cells. Clusters of Sertoli cells were observed in the lumen of the seminiferous tubules; impaired function of these cells could also prevent the complete recovery of the seminiferous epithelium. By 16 weeks after the end of irradiation 67% of 5 cGy/day irradiated rats and 34% of 7 cGy/day irradiated rats recovered fertility.
雄性斯普拉格-道利大鼠以每天5或7厘戈瑞的剂量率持续照射，直至总剂量达到900厘戈瑞。研究了照射过程中精子发生的变化以及照射后33周内睾丸的恢复情况。睾丸重量未出现明显的剂量率效应。在照射期间，剂量和剂量率的增加导致A型精原细胞和前细线期精母细胞数量减少。在我们的实验条件下，生殖细胞的产生并未达到平台期，超过500厘戈瑞后，无生殖细胞的管状横截面积增加就表明了这一点。生精上皮的恢复主要在9周内发生。它不依赖于剂量率，33周后仍未完全恢复。这种恢复不足可能是由于干细胞的代偿分裂能力有限。在生精小管腔内观察到支持细胞簇；这些细胞功能受损也可能阻止生精上皮的完全恢复。照射结束后16周，每天接受5厘戈瑞照射的大鼠中有67%恢复生育能力，每天接受7厘戈瑞照射的大鼠中有34%恢复生育能力。
