Academy of Agriculture and Forestry Sciences, Qinghai University, Xining, 810016, China.
State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, China.
Sci Rep. 2024 Jul 2;14(1):15228. doi: 10.1038/s41598-024-66041-w.
In order to resolve the key genes for weed control by Trichoderma polysporum at the genomic level, we extracted the genomic DNA and sequenced the whole genome of T. polysporum strain HZ-31 on the Illumina Hiseq platform. The raw data was cleaned up using Trimmomatic and checked for quality using FastQC. The sequencing data was assembled using SPAdes, and GeneMark was used to perform gene prediction on the assembly results. The results showed that the genome size of T. polysporum HZ-31 was 39,325,746 bp, with 48% GC content, and the number of genes encoded was 11,998. A total of 148 tRNAs and 45 rRNAs were predicted. A total of 782 genes were annotated in the Carbohydrase Database, 757 genes were annotated to the Pathogen-Host Interaction Database, and 67 gene clusters were identified. In addition, 1023 genes were predicted to be signal peptide proteins. The annotation and functional analysis of the whole genome sequence of T. polymorpha HZ-31 provide a basis for the in-depth study of the molecular mechanism of its herbicidal action and more effective utilization for weed control.
为了在基因组水平上解决木霉多孢菌防治杂草的关键基因问题，我们提取了木霉多孢菌菌株 HZ-31 的基因组 DNA，并在 Illumina Hiseq 平台上对其全基因组进行了测序。使用 Trimmomatic 对原始数据进行了清理，并使用 FastQC 检查了质量。使用 SPAdes 对测序数据进行了组装，并使用 GeneMark 对组装结果进行了基因预测。结果表明，木霉多孢菌 HZ-31 的基因组大小为 39325746bp，GC 含量为 48%，编码的基因数量为 11998 个。共预测了 148 个 tRNA 和 45 个 rRNA。在碳水化合物酶数据库中总共注释了 782 个基因，在病原体-宿主相互作用数据库中注释了 757 个基因，鉴定了 67 个基因簇。此外，预测了 1023 个信号肽蛋白基因。木霉多孢菌 HZ-31 全基因组序列的注释和功能分析为深入研究其除草作用的分子机制和更有效地利用其防治杂草提供了基础。
