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[运用直接法和血清学法检测女性子宫颈沙眼衣原体感染情况]

[Use of direct and serologic methods to detect Chlamydia trachomatis infections in cervix uteri of women].

作者信息

Kazanowska W, Zdrodowska-Stefanow B, Kuczyńska K

机构信息

Instytut Połoznictwa i Chorób Kobiecych.

出版信息

Med Dosw Mikrobiol. 1993;45(3):349-55.

PMID:8189809
Abstract

Investigation included 254 women (18-75 years of age). In this number, 74 patients constituted a group with pathological changes in cervix uteri, while 180 women were seemingly healthy. The material was evaluated by a method of direct immunofluorescence (IF) with monoclonal antibody serum and a culture on McCoy cells. Antibodies in blood serum was detected by an immunoenzymatic test ELISA. Out of total number of 256 patients tested, elementary bodies of C. trachomatis were found in cylindrical cells of cervix uteri by the IF test in 54 (21.25%) of women. Analogical percentage of positive results was obtained by application of culture on McCoy cells. Positive serological results were found in 70 patients (27.55%). In group of 75 patients infected with C. trachomatis, 33 cases (44.59%) were confirmed by application of IF and cell culture, whereas positive serological results were found in 39 (52.70%) of investigated patients. In the group of seemingly healthy women in 11.60% elementary bodies of C. trachomatis were detected and in 17.22% of cases serological results were positive.

摘要

研究纳入了254名女性(年龄在18至75岁之间)。其中,74例患者构成子宫颈有病理变化的一组,而180名女性看似健康。通过使用单克隆抗体血清的直接免疫荧光法(IF)和在 McCoy 细胞上进行培养来评估材料。血清中的抗体通过免疫酶联试验ELISA进行检测。在总共256名接受检测的患者中,通过IF试验在54名(21.25%)女性的子宫颈柱状细胞中发现了沙眼衣原体的原体。通过在 McCoy 细胞上进行培养获得了类似的阳性结果百分比。70名患者(27.55%)的血清学结果呈阳性。在75名感染沙眼衣原体的患者组中,33例(44.59%)通过IF和细胞培养得到证实,而在39名(52.70%)受调查患者中血清学结果呈阳性。在看似健康的女性组中,检测到11.60%的沙眼衣原体原体,17.22%的病例血清学结果呈阳性。

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