State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen, Fujian, China.
State-Province Joint Engineering Laboratory of Marine Bioproducts and Technology, College of Ocean and Earth Sciences, Xiamen University, Xiamen, Fujian, China.
BMC Microbiol. 2024 Jul 3;24(1):239. doi: 10.1186/s12866-024-03398-y.
The gut microbiota significantly influences the health and growth of red-spotted grouper (Epinephelus akaara), a well-known commercial marine fish from Fujian Province in southern China. However, variations in survival strategies and seasons can impact the stability of gut microbiota data, rendering it inaccurate in reflecting the state of gut microbiota. Which impedes the effective enhancement of aquaculture health through a nuanced understanding of gut microbiota. Inspired by this, we conducted a comprehensive analysis of the gut microbiota of wild and captive E. akaara in four seasons.
Seventy-two E. akaara samples were collected from wild and captive populations in Dongshan city, during four different seasons. Four sections of the gut were collected to obtain comprehensive information on the gut microbial composition and sequenced using 16S rRNA next-generation Illumina MiSeq. We observed the highest gut microbial diversity in both captive and wild E. akaara during the winter season, and identified strong correlations with water temperature using Mantel analysis. Compared to wild E. akaara, we found a more complex microbial network in captive E. akaara, as evidenced by increased abundance of Bacillaceae, Moraxellaceae and Enterobacteriaceae. In contrast, Vibrionaceae, Clostridiaceae, Flavobacteriaceae and Rhodobacteraceae were found to be more active in wild E. akaara. However, some core microorganisms, such as Firmicutes and Photobacterium, showed similar distribution patterns in both wild and captive groups. Moreover, we found the common community composition and distribution characteristics of top 10 core microbes from foregut to hindgut in E. akaara.
Collectively, the study provides relatively more comprehensive description of the gut microbiota in E. akaara, taking into account survival strategies and temporal dimensions, which yields valuable insights into the gut microbiota of E. akaara and provides a valuable reference to its aquaculture.
肠道微生物群对红鳍东方鲀（Epinephelus akaara）的健康和生长有重要影响，红鳍东方鲀是中国南方福建省著名的商业海水鱼类。然而，生存策略和季节的变化会影响肠道微生物群数据的稳定性，使其无法准确反映肠道微生物群的状态。这阻碍了通过对肠道微生物群的细致了解来有效提高水产养殖健康水平。受此启发，我们对四个季节的野生和圈养红鳍东方鲀的肠道微生物群进行了全面分析。
从东山县的野生和圈养种群中收集了 72 个红鳍东方鲀样本，分别来自四个不同的季节。采集了肠道的四个部分，以获得肠道微生物组成的综合信息，并使用 16S rRNA 下一代 Illumina MiSeq 进行测序。我们观察到冬季野生和圈养红鳍东方鲀的肠道微生物多样性最高，并通过 Mantel 分析发现与水温有很强的相关性。与野生红鳍东方鲀相比，我们发现圈养红鳍东方鲀的微生物网络更为复杂，Bacillaceae、Moraxellaceae 和 Enterobacteriaceae 的丰度增加。相比之下，Vibrionaceae、Clostridiaceae、Flavobacteriaceae 和 Rhodobacteraceae 在野生红鳍东方鲀中更为活跃。然而，一些核心微生物，如 Firmicutes 和 Photobacterium，在野生和圈养组中表现出相似的分布模式。此外，我们还发现了红鳍东方鲀前肠到后肠的 10 种核心微生物的共同群落组成和分布特征。
总的来说，该研究考虑了生存策略和时间维度，对红鳍东方鲀的肠道微生物群进行了相对更全面的描述，为红鳍东方鲀的肠道微生物群提供了有价值的见解，并为其水产养殖提供了有价值的参考。
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