Montalvo-Fernández Grecia, Ortiz-Alcantara Joanna M, Durruty-Lagunes Claudia, Espinosa-Asuar Laura, Reyes-Sosa Mariela Beatriz, Arena-Ortiz María Leticia
Laboratorio de Estudios Ecogenómicos, Facultad de Ciencias, Universidad Nacional Autónoma de México (UNAM), Parque Científico Tecnológico de Yucatán, Carretera Sierra Papacal-Chuburná Puerto, Km, 5.5, Tablaje 31257, Sierra Papacal, Mérida 97302, Yucatán, Mexico.
Unidad Multidisciplinaria de Docencia e Investigación (UMDI) Sisal, Facultad de Ciencias, Universidad Nacional Autónoma de México (UNAM), Puerto de Abrigo S/N, Sisal 97355, Yucatán, Mexico.
Microorganisms. 2025 Jul 31;13(8):1792. doi: 10.3390/microorganisms13081792.
The gut microbiota plays an essential role in the host's metabolism. Its composition and structure depend on biological and environmental factors. This work was designed to identify the composition and structure of the wild and captive red grouper () microbiota and make predictions regarding its metabolic functions. Our hypothesis stated that wild and captive individuals would share the most abundant taxonomic groups, forming a core microbiota, and individuals in captivity might have exclusive taxonomic groups. Metagenomic DNA was extracted from the intestinal contents of wild and captive individuals. The 16S rRNA gene was amplified and sequenced using Illumina pair-end technology. QIIME2 pipeline was used for sequence analysis and alpha and beta diversity assessment. PICRUSt was used to infer metabolic functions. Twenty-nine phyla were identified; the most abundant were Pseudomonadota, Bacillota, Fusobacteriota, and Actinomycetota. The dominant genera were , , , and . The metabolic prediction analysis suggested that the gut microbiota is related to food digestion, the immune system, antioxidant enzymes, antibiotic resistance, and vitamin B12 transport. We concluded that the microbiota of established in captivity is sensitive to environmental changes such as water pollution, which can cause a decrease in diversity.
肠道微生物群在宿主新陈代谢中起着至关重要的作用。其组成和结构取决于生物和环境因素。本研究旨在确定野生和圈养红斑石斑鱼（）微生物群的组成和结构，并预测其代谢功能。我们的假设是，野生和圈养个体将共享最丰富的分类群，形成核心微生物群，而圈养个体可能有独特的分类群。从野生和圈养个体的肠道内容物中提取宏基因组DNA。使用Illumina双端技术对16S rRNA基因进行扩增和测序。QIIME2管道用于序列分析以及α和β多样性评估。PICRUSt用于推断代谢功能。共鉴定出29个门；最丰富的是假单胞菌门、芽孢杆菌门、梭杆菌门和放线菌门。优势属为……。代谢预测分析表明，肠道微生物群与食物消化、免疫系统、抗氧化酶、抗生素抗性和维生素B12转运有关。我们得出结论，圈养条件下建立的微生物群对水污染等环境变化敏感，这可能导致多样性降低。
