酵母甾醇营养缺陷型酵母质膜组成随外源甾醇的调节作用。

Modulation of yeast plasma membrane composition of a yeast sterol auxotroph as a function of exogenous sterol.

作者信息

Low C, Rodriguez R J, Parks L W

出版信息

Arch Biochem Biophys. 1985 Aug 1;240(2):530-8. doi: 10.1016/0003-9861(85)90059-1.

Abstract

Plasma membranes isolated from a yeast sterol auxotroph (RD5-R) grown on 1, 5, and 15 micrograms ml-1 exogenous concentrations of sterol showed no discontinuity in plots of steady-state fluorescence anisotropy. Liposomes constructed from phospholipid and sterol extracted from RD5-R grown on different sterols indicated that exogenously supplied sterol modulated cellular phospholipids such that lipid-phase transitions were avoided. Liposomes derived from sterol and phospholipid extracted from the same culture exhibited no lipid-phase transitions. However, when phospholipid extracted from a culture grown on a specific sterol was mixed with sterol extracted from a heterologous culture grown on a different sterol to form liposomes, discontinuities were detected in the anisotropy measurements of the liposomes produced. Quantitative analyses revealed that the exogenously supplied sterol coordinately regulated specific phospholipid species, fatty acid composition, and sterol to phospholipid ratios in yeast auxotrophs.

摘要

从在1、5和15微克/毫升外源甾醇浓度下生长的酵母甾醇营养缺陷型（RD5 - R）中分离出的质膜，在稳态荧光各向异性图中未显示出不连续性。由从在不同甾醇上生长的RD5 - R中提取的磷脂和甾醇构建的脂质体表明，外源提供的甾醇调节细胞磷脂，从而避免脂质相转变。源自同一培养物中提取的甾醇和磷脂的脂质体未表现出脂质相转变。然而，当从在特定甾醇上生长的培养物中提取的磷脂与从在不同甾醇上生长的异源培养物中提取的甾醇混合以形成脂质体时，在产生的脂质体的各向异性测量中检测到不连续性。定量分析表明，外源提供的甾醇协同调节酵母营养缺陷型中特定的磷脂种类、脂肪酸组成以及甾醇与磷脂的比例。