酿酒酵母膜固醇修饰对乙醇存在下生长的响应。
Saccharomyces cerevisiae membrane sterol modifications in response to growth in the presence of ethanol.
作者信息
Walker-Caprioglio H M, Casey W M, Parks L W
机构信息
Department of Microbiology, North Carolina State University, Raleigh 27695-7615.
出版信息
Appl Environ Microbiol. 1990 Sep;56(9):2853-7. doi: 10.1128/aem.56.9.2853-2857.1990.
Abstract
Membranes isolated from yeasts grown in the presence of ethanol do not display the thermally induced transition in diphenylhexatriene anisotropy that is seen in control cells when they are exposed to ethanol in vitro. The total sterol content of the cells that were exposed to ethanol during growth is reduced, with no steryl esters being detected. A greater proportion of the total sterol pool is ergosterol in cells grown in the presence of alcohol. The activity of 3-hydroxy-3-methylglutaryl coenzyme A reductase is reduced by ethanol in vitro. Ethanol-exposed cells take up more exogenous sterol under aerobic conditions than do control cells. The presence of ethanol during growth reduces the activity of the plasma membrane enzyme, chitin synthase, as well as increasing the thermosensitivity of this enzyme.
摘要
从在乙醇存在下生长的酵母中分离出的膜，在体外暴露于乙醇时，不会表现出对照细胞中观察到的二苯基己三烯各向异性的热诱导转变。在生长过程中暴露于乙醇的细胞的总甾醇含量降低，未检测到甾醇酯。在酒精存在下生长的细胞中，总甾醇库中麦角固醇的比例更大。体外乙醇会降低3-羟基-3-甲基戊二酰辅酶A还原酶的活性。在有氧条件下，暴露于乙醇的细胞比对照细胞摄取更多的外源性甾醇。生长过程中乙醇的存在会降低质膜酶几丁质合酶的活性，并增加该酶的热敏感性。
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