School of Environment, Liaoning University, Shenyang, 110036, China.
Sci Rep. 2024 Jul 4;14(1):15420. doi: 10.1038/s41598-024-66399-x.
Due to the low permeability characteristics of the deep gas-containing coal seam, the conventional prevention and control measures that cannot solve the problems of gas outbursts are unsatisfactory for the prevention and control of the coal and gas outbursts disaster. Therefore, in this study, a strain of methane-oxidizing bacteria M with high-pressure resistance, strong resistance, and high methane degradation rate was selected from coal mines. The growth and degradation abilities of M in chelating wetting agent solutions to assess its adaptability and find the optimal agent-to-M07 ratio. It provides a new method for integrating the reduction of impact tendency and gas pressure in deep coal mines. The experimental results show that M is a Gram-positive bacterium of the genus Bacillus, which has strong resistance and adaptability to high-pressure water injection. By degrading 70 mol of methane, M produces 1 mol of carbon dioxide, which can reduce gas pressure and reduce the risk of gas outbursts in coal mines. As the experiment proves, the best effect was achieved when the M concentration of the chelating wetting agent was 0.05%. The methane-oxidizing bacteria based on the chelating wetting agent as carriers prove a new prevention and control method for the integrated prevention and control of coal and gas outbursts in coal mines and also provide a new idea for microbial application in coal mine disaster control.
由于深部含气煤层渗透率低，常规的防治措施无法解决瓦斯突出问题，因此对于煤与瓦斯突出灾害的防治效果不理想。因此，本研究从煤矿中选择了一株耐高压、抗强、甲烷降解率高的甲烷氧化菌 M。评估 M 在螯合润湿剂溶液中的生长和降解能力，以评估其适应性，并找到最佳的试剂与 M07 的比例。为深部煤矿中冲击倾向和瓦斯压力的降低提供了一种新的方法。实验结果表明，M 是芽孢杆菌属的革兰氏阳性菌，对高压注水具有很强的抵抗力和适应性。通过降解 70 mol 的甲烷，M 产生 1 mol 的二氧化碳，可降低瓦斯压力，降低煤矿瓦斯突出的风险。实验证明，当螯合润湿剂中的 M 浓度为 0.05%时，效果最佳。基于螯合润湿剂的甲烷氧化菌为煤矿的煤与瓦斯突出综合治理提供了一种新的防治方法，也为微生物在煤矿灾害防治中的应用提供了新的思路。
