杭州市儿童急性呼吸道感染中人副流感病毒 3 型的分子流行病学研究。

Molecular Epidemiology of Human Parainfluenza Virus Type 3 in Children With Acute Respiratory Tract Infection in Hangzhou.

机构信息

Department of Clinical Laboratory, The Children's Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Department of Infectious Diseases, Fujian Children's Hospital (Fujian Branch of Shanghai Children's Medical Center), National Regional Medical Center, College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Fuzhou, China.

出版信息

Influenza Other Respir Viruses. 2024 Jul;18(7):e13351. doi: 10.1111/irv.13351.

DOI:10.1111/irv.13351

PMID:38965872

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11224502/

Abstract

BACKGROUND

Since the outbreak of COVID-19, China has undertaken a variety of preventative and control measures, effectively reducing the incidence of numerous infectious diseases among the pediatric population in Hangzhou. We aim to investigate the genetic and epidemiological characteristics of Human parainfluenza virus-3 (HPIV-3) in pediatric patients during this period.

METHODS

A total of 1442 pharyngeal swab samples were collected from outpatients and inpatients with a diagnosis of acute respiratory tract infections (ARTIs) from November 2020 to March 2021. HPIV-3 was detected by quantitative real time polymerase chain reaction (qRT-PCR). The L gene of HPIV-3 positive samples was amplified and sequenced.

RESULTS

Among 1442 children with ARTI, the positive rate of HPIV-3 was 7.07% (102/1442). The positive detection rate was the highest in the 6-month to 1-year age group. Coinfection was observed in 36 HPIV-3-positive samples (35.29%, 36/102), and adenovirus (ADV) was the most common coinfecting virus (63.89%, 23/36). The L gene of 48 HPIV-3 positive samples was sequenced. The nucleotide sequence analysis showed high consistency (92.10%-99.40%), and all strains belonged to C3a.

CONCLUSIONS

During study periods, the positive detection rate of HPIV-3 among children is high, and the highest proportion of coinfection was observed in HPIV-3 mixed ADV infection. Phylogenetic analysis revealed that the nucleotide sequence of the L gene of HPIV-3 was highly consistent, and the main epidemic strain in this area was the C3a subtype.

摘要

背景

自 COVID-19 爆发以来，中国采取了多种防控措施，有效降低了杭州市儿科人群多种传染病的发病率。本研究旨在分析该时期小儿人副流感病毒 3 型（HPIV-3）的遗传和流行病学特征。

方法

收集 2020 年 11 月至 2021 年 3 月期间杭州市门诊和住院诊断为急性呼吸道感染（ARTI）的 1442 例咽拭子样本，采用实时荧光定量 PCR（qRT-PCR）检测 HPIV-3。对 HPIV-3 阳性样本的 L 基因进行扩增和测序。

结果

在 1442 例 ARTI 患儿中，HPIV-3 的阳性率为 7.07%（102/1442）。6 月龄至 1 岁年龄组的阳性检出率最高。在 36 例 HPIV-3 阳性样本中观察到混合感染（35.29%，36/102），最常见的混合感染病毒是腺病毒（ADV）（63.89%，23/36）。对 48 例 HPIV-3 阳性样本的 L 基因进行测序。核苷酸序列分析显示高度一致性（92.10%-99.40%），所有毒株均属于 C3a 亚群。