Hoven Darius, Inaoka Misaki, McCoy Reece, Withers Aimee, Owens Róisín M, Malliaras George G
Electrical Engineering Division, Department of Engineering, University of Cambridge, Cambridge, CB3 0FA UK.
Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, CB3 0AS UK.
MRS Commun. 2024;14(3):261-266. doi: 10.1557/s43579-024-00554-3. Epub 2024 Apr 22.
Microelectrode arrays (MEAs) have applications in drug discovery, toxicology, and basic research. They measure the electrophysiological response of tissue cultures to quantify changes upon exposure to biochemical stimuli. Unfortunately, manual addition of chemicals introduces significant noise in the recordings. Here, we report a simple-to-fabricate fluidic system that addresses this issue. We show that cell cultures can be successfully established in the fluidic compartment under continuous flow conditions and that the addition of chemicals introduces minimal noise in the recordings. This dynamic cell culture system represents an improvement over traditional tissue culture wells used in MEAs, facilitating electrophysiology measurements.
微电极阵列（MEA）在药物发现、毒理学和基础研究中都有应用。它们通过测量组织培养物的电生理反应来量化暴露于生化刺激后的变化。不幸的是，手动添加化学物质会在记录中引入大量噪声。在此，我们报告了一种易于制造的流体系统，该系统解决了这一问题。我们证明，在连续流动条件下，可以在流体隔室中成功建立细胞培养物，并且添加化学物质在记录中引入的噪声最小。这种动态细胞培养系统相较于MEA中使用的传统组织培养孔有了改进，有助于电生理学测量。
