Cancer Minimally Invasive Therapies Centre, Guangdong Second Provincial General Hospital, Jinan University, Guangzhou, Guangdong, 510632, China.
Department of Cell Biology & Institute of Biomedicine, Guangdong Provincial Biotechnology & Engineering Technology Research Center, Guangdong Provincial Key Laboratory of Bioengineering Medicine, Genomic Medicine Engineering Research Center of Ministry of Education, MOE Key Laboratory of Tumor Molecular Biology, National Engineering Research Center of Genetic Medicine, State Key Laboratory of Bioactive Molecules and Druggability Assessment, College of Life Science and Technology, Jinan University, Guangzhou, Guangdong, 510632, China.
Cell Death Dis. 2024 Jul 5;15(7):483. doi: 10.1038/s41419-024-06869-2.
Hepatocellular carcinoma (HCC) is one of the most common malignant tumors, and the expression and function of an uncharacterized protein RNF214 in HCC are still unknown. Phase separation has recently been observed to participate in the progression of HCC. In this study, we investigated the expression, function, and phase separation of RNF214 in HCC. We found that RNF214 was highly expressed in HCC and associated with poor prognosis. RNF214 functioned as an oncogene to promote the proliferation, migration, and metastasis of HCC. Mechanically, RNF214 underwent phase separation, and the coiled-coil (CC) domain of RNF214 mediated its phase separation. Furthermore, the CC domain was necessary for the oncogenic function of RNF214 in HCC. Taken together, our data favored that phase separation of RNF214 promoted the progression of HCC. RNF214 may be a potential biomarker and therapeutic target for HCC.
肝细胞癌（HCC）是最常见的恶性肿瘤之一，但其特征不明的蛋白 RNF214 在 HCC 中的表达和功能仍不清楚。最近观察到相分离参与 HCC 的进展。在这项研究中，我们研究了 RNF214 在 HCC 中的表达、功能和相分离。我们发现 RNF214 在 HCC 中高表达，并与预后不良相关。RNF214 作为癌基因促进 HCC 的增殖、迁移和转移。机制上，RNF214 发生相分离，RNF214 的卷曲螺旋（CC）结构域介导其相分离。此外，CC 结构域是 RNF214 在 HCC 中致癌功能所必需的。总之，我们的数据支持 RNF214 的相分离促进了 HCC 的进展。RNF214 可能是 HCC 的一个有潜力的生物标志物和治疗靶点。
