Buckley Alison, Guo Chang, Laycock Adam, Cui Xianjin, Belinga-Desaunay-Nault Marie-France, Valsami-Jones Eugenia, Leonard Martin, Smith Rachel
Toxicology Department, Radiation, Chemical and Environmental Hazards Directorate (RCE), UK Health Security Agency (UKHSA), Harwell Campus, Oxfordshire OX11 0RQ, UK; The National Institute for Health Research Health Protection Research Unit (NIHR HPRU) in Environmental Exposures and Health (EEH) at Imperial College London in Partnership with UKHSA, School of Public Health, Imperial College London, Michael Uren Biomedical Engineering Hub, White City Campus, Wood Lane, W12 OBZ, UK.
School of Geography, Earth and Environmental Sciences, University of Birmingham, Birmingham B15 2TT, UK; Nanodot Limited, Loughborough LE11 4NT, UK.
Toxicol In Vitro. 2024 Oct;100:105889. doi: 10.1016/j.tiv.2024.105889. Epub 2024 Jul 5.
Experimental systems allowing aerosol exposure (AE) of cell cultures at the air-liquid-interface (ALI) are increasingly being used to assess the toxicity of inhaled contaminants as they are more biomimetic than standard methods using submerged cultures, however, they require detailed characterisation before use. An AE-ALI system combining aerosol generation with a CULTEX® exposure chamber was characterised with respect to particle deposition and the cellular effects of filtered air (typical control) exposures. The effect of system parameters (electrostatic precipitator voltage, air flowrate to cells and insert size) on deposition efficiency and spatial distribution were investigated using ICP-MS and laser ablation ICP-MS, for an aerosol of CeO nanoparticles. Deposition varied with conditions, but appropriate choice of operating parameters produced broadly uniform deposition at suitable levels. The impact of air exposure duration on alveolar cells (A549) and primary small airway epithelial cells (SAECs) was explored with respect to LDH release and expression of selected genes. Results indicated that air exposures could have a significant impact on cells (e.g., cytotoxicity and expression of genes, including CXCL1, HMOX1, and SPP1) at relatively short durations (from 10 mins) and that SAECs were more sensitive. These findings indicate that detailed system characterisation is essential to ensure meaningful results.
允许在气液界面（ALI）对细胞培养物进行气溶胶暴露（AE）的实验系统越来越多地用于评估吸入污染物的毒性，因为它们比使用浸没培养的标准方法更具仿生学特性，然而，在使用前需要进行详细的表征。结合气溶胶生成与CULTEX®暴露室的AE-ALI系统，针对颗粒沉积和过滤空气（典型对照）暴露的细胞效应进行了表征。使用ICP-MS和激光烧蚀ICP-MS，针对CeO纳米颗粒气溶胶，研究了系统参数（静电除尘器电压、流向细胞的空气流速和插入物尺寸）对沉积效率和空间分布的影响。沉积随条件而变化，但通过适当选择操作参数，可以在合适的水平上实现大致均匀的沉积。针对乳酸脱氢酶（LDH）释放和选定基因的表达，探讨了空气暴露持续时间对肺泡细胞（A549）和原代小气道上皮细胞（SAECs）的影响。结果表明，在相对较短的持续时间（从10分钟起），空气暴露可能会对细胞产生重大影响（例如细胞毒性和包括CXCL1、HMOX1和SPP1在内的基因表达），并且SAECs更敏感。这些发现表明，详细的系统表征对于确保获得有意义的结果至关重要。
