Differences in cytotoxicity of lung epithelial cells exposed to titanium dioxide nanofibers and nanoparticles: Comparison of air-liquid interface and submerged cell cultures.

Air Liquid Interface (ALI) system has emerged as a useful tool for toxicity evaluation of nanomaterials related to inhalation since the system mimics the aerosol exposure. We compared the biological responses of lung epithelial cells exposed to titanium dioxide (TiO) nanofibers and nanoparticles in ALI and submerged cell cultures systems. Cells were exposed to 2 and 10 μg/cm for 24 h, 48 h and 72 h and LDH release, TiO internalization, DNA-double strand breaks (DSBs) and ROS production were assessed. LDH release was similar in both systems and particles had higher cytoplasmic uptake in submerged systems. Both TiO types were located in the cytoplasm but nanofibers had nuclear uptake regardless to the system tested. Cells exposed to TiO nanofibers had higher DSBs in the ALI system than in submerged cell cultures but cells exposed to TiO nanoparticles had similar DSBs in both systems. ROS production was higher in cells exposed to TiO nanofibers compared to cells exposed to TiO nanoparticles. In conclusion, cytotoxicity of lung epithelial cells was similar in ALI or submerged cell cultures, however cells exposed to TiO nanofibers displayed higher toxicity than cells exposed to TiO nanoparticles.