Latvala Siiri, Hedberg Jonas, Möller Lennart, Odnevall Wallinder Inger, Karlsson Hanna L, Elihn Karine
Department of Environmental Science and Analytical Chemistry, Atmospheric Science Unit, Stockholm University, SE-106 91, Stockholm, Sweden.
Division of Surface and Corrosion Science, School of Chemical Science and Engineering, KTH Royal Institute of Technology, SE-100 44, Stockholm, Sweden.
J Appl Toxicol. 2016 Oct;36(10):1294-301. doi: 10.1002/jat.3304. Epub 2016 Mar 3.
The use of refined toxicological methods is currently needed for characterizing the risks of airborne nanoparticles (NPs) to human health. To mimic pulmonary exposure, we have developed an air-liquid interface (ALI) exposure system for direct deposition of airborne NPs on to lung cell cultures. Compared to traditional submerged systems, this allows more realistic exposure conditions for characterizing toxicological effects induced by airborne NPs. The purpose of this study was to investigate how the deposition of silver NPs (AgNPs) is affected by different conditions of the ALI system. Additionally, the viability and metabolic activity of A549 cells was studied following AgNP exposure. Particle deposition increased markedly with increasing aerosol flow rate and electrostatic field strength. The highest amount of deposited particles (2.2 μg cm(-2) ) at cell-free conditions following 2 h exposure was observed for the highest flow rate (390 ml min(-1) ) and the strongest electrostatic field (±2 kV). This was estimated corresponding to deposition efficiency of 94%. Cell viability was not affected after 2 h exposure to clean air in the ALI system. Cells exposed to AgNPs (0.45 and 0.74 μg cm(-2) ) showed significantly (P < 0.05) reduced metabolic activities (64 and 46%, respectively). Our study shows that the ALI exposure system can be used for generating conditions that were more realistic for in vitro exposures, which enables improved mechanistic and toxicological studies of NPs in contact with human lung cells.Copyright © 2016 The Authors Journal of Applied Toxicology Published by John Wiley & Sons Ltd.
目前需要使用精细的毒理学方法来表征空气中纳米颗粒（NPs）对人类健康的风险。为模拟肺部暴露，我们开发了一种气液界面（ALI）暴露系统，用于将空气中的NPs直接沉积到肺细胞培养物上。与传统的浸没系统相比，这为表征空气中NPs诱导的毒理学效应提供了更真实的暴露条件。本研究的目的是调查ALI系统的不同条件如何影响银纳米颗粒（AgNPs）的沉积。此外，还研究了AgNP暴露后A549细胞的活力和代谢活性。颗粒沉积随着气溶胶流速和静电场强度的增加而显著增加。在2小时暴露后的无细胞条件下，对于最高流速（390 ml min(-1)）和最强静电场（±2 kV），观察到最高沉积颗粒量（2.2 μg cm(-2)）。估计这对应于94%的沉积效率。在ALI系统中暴露于清洁空气2小时后，细胞活力未受影响。暴露于AgNPs（0.45和0.74 μg cm(-2)）的细胞显示出代谢活性显著降低（分别为64%和46%，P < 0.05）。我们的研究表明，ALI暴露系统可用于生成更适合体外暴露的条件，从而能够改进对与人类肺细胞接触的NPs的机制和毒理学研究。版权所有© 2016作者《应用毒理学杂志》由John Wiley & Sons Ltd出版。
