Ďúranová Hana, Kšiňan Samuel, Kuželová Lenka, Šimora Veronika, Ďurišová Ľuba, Olexíková Lucia, Ernst Dávid, Kolenčík Marek
AgroBioTech Research Centre, Slovak University of Agriculture, Tr. A. Hlinku 2, 949 76, Nitra, Slovakia.
Institute of Plant and Environmental Sciences, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 94976, Nitra, Slovakia.
Chemosphere. 2024 Sep;363:142772. doi: 10.1016/j.chemosphere.2024.142772. Epub 2024 Jul 4.
Ensuring global food security is pressing among challenges like population growth, climate change, soil degradation, and diminishing resources. Meeting the rising food demand while reducing agriculture's environmental impact requires innovative solutions. Nanotechnology, with its potential to revolutionize agriculture, offers novel approaches to these challenges. However, potential risks and regulatory aspects of nanoparticle (NP) utilization in agriculture must be considered to maximize their benefits for human health and the environment. Understanding NP-plant cell interactions is crucial for assessing risks of NP exposure and developing strategies to control NP uptake by treated plants. Insights into NP uptake mechanisms, distribution patterns, subcellular accumulation, and induced alterations in cellular architecture can be effectively drawn using transmission electron microscopy (TEM). TEM allows direct visualization of NPs within plant tissues/cells and their influence on organelles and subcellular structures at high resolution. Moreover, integrating TEM with stereological principles, which has not been previously utilized in NP-plant cell interaction assessments, provides a novel and quantitative framework to assess these interactions. Design-based stereology enhances TEM capability by enabling precise and unbiased quantification of three-dimensional structures from two-dimensional images. This combined approach offers comprehensive data on NP distribution, accumulation, and effects on cellular morphology, providing deeper insights into NP impact on plant physiology and health. This report highlights the efficient use of TEM, enhanced by stereology, in investigating diverse NP-plant tissue/cell interactions. This methodology facilitates detailed visualization of NPs and offers robust quantitative analysis, advancing our understanding of NP behavior in plant systems and their potential implications for agricultural sustainability.
在人口增长、气候变化、土壤退化和资源减少等挑战中，确保全球粮食安全迫在眉睫。在满足不断增长的粮食需求的同时减少农业对环境的影响，需要创新的解决方案。纳米技术有潜力彻底改变农业，为这些挑战提供了新方法。然而，必须考虑纳米颗粒（NP）在农业中应用的潜在风险和监管方面，以最大限度地提高其对人类健康和环境的益处。了解NP与植物细胞的相互作用对于评估NP暴露风险和制定控制处理过的植物对NP吸收的策略至关重要。利用透射电子显微镜（TEM）可以有效地深入了解NP的吸收机制、分布模式、亚细胞积累以及诱导的细胞结构变化。TEM能够在高分辨率下直接观察植物组织/细胞内的NP及其对细胞器和亚细胞结构的影响。此外，将TEM与体视学原理相结合，这在以前的NP-植物细胞相互作用评估中尚未使用过，提供了一个新颖的定量框架来评估这些相互作用。基于设计的体视学通过从二维图像中精确且无偏地量化三维结构来增强TEM的能力。这种结合的方法提供了关于NP分布、积累以及对细胞形态影响的全面数据，更深入地了解NP对植物生理和健康的影响。本报告强调了通过体视学增强的TEM在研究各种NP-植物组织/细胞相互作用中的有效应用。这种方法有助于对NP进行详细的可视化观察，并提供强大的定量分析，加深我们对NP在植物系统中的行为及其对农业可持续性潜在影响的理解。
