Rabinovitch A, Russell T, Mintz D H
Diabetes. 1979 Dec;28(12):1108-13. doi: 10.2337/diab.28.12.1108.
A possible role for fibroblasts in promoting the survival and function of islet B cells in tissue culture was examined by the addition of fibroblasts from a mouse embryo cell line (3T3-L2) to islet cell monolayer cultures prepared from newborn rat pancreases. Co-culture of islet cells with fibroblasts significantly increased the recovery of insulin in medium and cells after 7 days of culture in medium supplemented with 10% serum, and prevented the deterioration of islet cells cultured in serum-free medium. Similarly- serum-free medium, conditioned by cultures of either 3T3-L2 fibroblasts or fibroblasts freshly isolated from newborn rat pancreases, maintained the release and content of insulin in islet cell monolayer cultures at levels four- to eightfold higher than in control serum-free medium. Serum-free, fibroblast-conditioned medium also enhanced the survival of intact islets maintained in free-floating culture for 28 days. The active factor(s) in fibroblast-conditioned medium has a high molecular weight and is heat-stable. We conclude that fibroblastic cells produce a macromolecular factor(s) capable of enhancing the survival of functional islet B cells in tissue culture.
通过将源自小鼠胚胎细胞系（3T3-L2）的成纤维细胞添加到由新生大鼠胰腺制备的胰岛细胞单层培养物中，研究了成纤维细胞在促进组织培养中胰岛B细胞存活和功能方面可能发挥的作用。在补充有10%血清的培养基中培养7天后，胰岛细胞与成纤维细胞共培养显著提高了培养基和细胞中胰岛素的回收率，并防止了在无血清培养基中培养的胰岛细胞的退化。同样，由3T3-L2成纤维细胞培养物或从新生大鼠胰腺新鲜分离的成纤维细胞培养物所产生的条件无血清培养基，使胰岛细胞单层培养物中胰岛素的释放量和含量维持在比对照无血清培养基高4至8倍的水平。无血清的成纤维细胞条件培养基还提高了在自由漂浮培养中维持28天的完整胰岛的存活率。成纤维细胞条件培养基中的活性因子分子量高且热稳定。我们得出结论，成纤维细胞产生一种能够在组织培养中增强功能性胰岛B细胞存活的大分子因子。
