寡食性叶甲 Ophraella communa（鞘翅目：叶甲科）的染色体水平基因组组装。

Chromosome-level genome assembly of an oligophagous leaf beetle Ophraella communa (Coleoptera: Chrysomelidae).

机构信息

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.

Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China.

出版信息

Sci Data. 2024 Jul 6;11(1):735. doi: 10.1038/s41597-024-03486-8.

DOI:10.1038/s41597-024-03486-8

PMID:38971852

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11227576/

Abstract

The leaf beetle Ophraella communa LeSage (Coleoptera: Chrysomelidae) is an effective biological control agent of the common ragweed. Here, we assembled a chromosome-level genome of the O. communa by combining Illumina, Nanopore, and Hi-C sequencing technologies. The genome size of the final genome assembly is 733.1 Mb, encompassing 17 chromosomes, with an improved contig N50 of 7.05 Mb compared to the original version. Genome annotation reveals 25,873 protein-coding genes, with functional annotations available for 22,084 genes (85.35%). Non-coding sequence annotation identified 204 rRNAs, 626 tRNAs, and 1791 small RNAs. Repetitive elements occupy 414.41 Mb, constituting 57.76% of the genome. This high-quality genome is fundamental for advancing biological control strategies employing O. communa.

摘要

叶甲属昆虫 Ophraella communa LeSage（鞘翅目：叶甲科）是豚草的有效生物防治因子。在这里，我们通过结合 Illumina、Nanopore 和 Hi-C 测序技术，组装了 O. communa 的染色体水平基因组。最终基因组组装的大小为 733.1 Mb，包含 17 条染色体，与原始版本相比，改进后的 contig N50 为 7.05 Mb。基因组注释揭示了 25873 个蛋白质编码基因，其中 22084 个基因（85.35%）具有功能注释。非编码序列注释鉴定了 204 个 rRNA、626 个 tRNA 和 1791 个小 RNA。重复元件占据了 414.41 Mb，占基因组的 57.76%。这个高质量的基因组是利用 O. communa 推进生物防治策略的基础。