Ma Chao, Cui Shaowei, Tian Zhenya, Zhang Yan, Chen Guangmei, Gao Xuyuan, Tian Zhenqi, Chen Hongsong, Guo Jianying, Zhou Zhongshi
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
School of Plant Protection, Jilin Agricultural University, Changchun, China.
Front Physiol. 2019 Oct 15;10:1290. doi: 10.3389/fphys.2019.01290. eCollection 2019.
Chemosensory proteins (CSPs) are considered to be the transporter linking odorant chemicals and receptors on sensory neurons. However, the extensive expression patterns of CSPs in insects suggest that CSPs are also involved in other physiological processes; the range of their functions, however, remains uncertain. In this study, we successfully characterized and cloned the CSP12 of (). The open reading frame of encodes 131 amino acids, with four conserved cysteine residues. The expression patterns of validated by quantitative real-time PCR (qRT-PCR) showed that is specifically expressed in female ovary. Furthermore, compared with the control treatment, silencing resulted in significantly reduced oviposition in females. Surprisingly, the knock-down rate of exceeded 95% and remained depressed for more than 15 days, indicating that RNA interference (RNAi) was a suitable method for exploring the function of CSP12 in These findings increase our understanding of the expression profile and function of the CSP gene family in insects.
化学感受蛋白（CSPs）被认为是连接气味化学物质和感觉神经元上受体的转运蛋白。然而，CSPs在昆虫中的广泛表达模式表明，CSPs也参与其他生理过程；然而，它们的功能范围仍不确定。在本研究中，我们成功地鉴定并克隆了（）的CSP12。（）的开放阅读框编码131个氨基酸，有四个保守的半胱氨酸残基。通过定量实时PCR（qRT-PCR）验证的（）表达模式表明，（）在雌性卵巢中特异性表达。此外，与对照处理相比，沉默（）导致雌性产卵显著减少。令人惊讶的是，（）的敲低率超过95%，并在超过15天的时间内持续降低，表明RNA干扰（RNAi）是探索CSP12在（）中功能的合适方法。这些发现增加了我们对昆虫中CSP基因家族表达谱和功能的理解。
