使用选择性杜博斯培养基从山羊的肠黏膜和肠系膜淋巴结中分离副结核分枝杆菌。
Isolation of Mycobacterium paratuberculosis from intestinal mucosa and mesenteric lymph nodes of goats by use of selective Dubos medium.
作者信息
Saxegaard F
出版信息
J Clin Microbiol. 1985 Aug;22(2):312-3. doi: 10.1128/jcm.22.2.312-313.1985.
Abstract
To isolate Mycobacterium paratuberculosis from contaminated material, a selective medium, selective Dubos medium (SDubos), was developed by supplementing conventional Dubos medium (CDubos) with carbenicillin, polymyxin, trimethoprim, and amphotericin B. The intestine and mesenteric lymph nodes of 1,501 goats were cultured in parallel on SDubos and CDubos after decontamination with oxalic acid. The contamination rate was reduced more than 150 times by the use of SDubos. The number of positive specimens (18, or 1.2%), i.e., those which revealed growth of M. paratuberculosis, was too small to evaluate the number of specimens which could have been missed due to contamination on CDubos. However, the number of specimens positive on SDubos (16, or 89%) showed that the antibiotics used were not harmful to M. paratuberculosis.
摘要
为了从受污染材料中分离副结核分枝杆菌，通过在传统杜博斯培养基（CDubos）中添加羧苄青霉素、多粘菌素、甲氧苄啶和两性霉素B，开发了一种选择性培养基——选择性杜博斯培养基（SDubos）。在用草酸去污后，将1501只山羊的肠道和肠系膜淋巴结分别在SDubos和CDubos上进行平行培养。使用SDubos后污染率降低了150多倍。阳性标本（即显示副结核分枝杆菌生长的标本）数量为18个（占1.2%），太少以至于无法评估因CDubos上的污染而可能遗漏的标本数量。然而，在SDubos上呈阳性的标本数量为16个（占89%），这表明所用抗生素对副结核分枝杆菌无害。
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