一种用于从牛粪中培养副结核分枝杆菌的改良培养基。
An improved medium for culture of Mycobacterium paratuberculosis from bovine faeces.
作者信息
Jørgensen J B
出版信息
Acta Vet Scand. 1982;23(3):325-35. doi: 10.1186/BF03546784.
Abstract
Löwenstein-Jensen medium with mycobactin, cyclo-heximide, penicillin, and chloramphenicol, and enriched with sodium pyruvate, was compared with an ordinary L.-J. medium with mycobactin. Faeces samples from cattle experimentally infected with M. paratuberculosis were cultured on both media. The improved medium gave 11 % more positive cultures and 90 % more colonies. Of the positive cultures 97 % showed detectable growth after 8 weeks of incubation, and the contamination rate was reduced to 0.4 By culture of faeces samples from naturally infected cattle the improved medium identified 23.2 % more infected animals than the basic medium, mostly due to a reduction of the contamination rate to about 3 .
摘要
将含有分枝杆菌素、放线菌酮、青霉素和氯霉素并添加丙酮酸钠的改良罗氏培养基与含分枝杆菌素的普通罗氏培养基进行比较。用副结核分枝杆菌实验感染牛的粪便样本在这两种培养基上进行培养。改良培养基的阳性培养物多出11%，菌落多出90%。在阳性培养物中，97%在培养8周后出现可检测到的生长，污染率降至0.4%。通过对自然感染牛的粪便样本进行培养，改良培养基比基础培养基多鉴定出23.2%的感染动物，这主要是因为污染率降至约3%。
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本文引用的文献
1
Varieties of Mycobacterium johnei isolated from sheep.从绵羊中分离出的副结核分枝杆菌变种。
J Pathol Bacteriol. 1951 Apr;63(2):333-6. doi: 10.1002/path.1700630217.
2
Observations on the isolation of Mycobacterium johnei in primary culture.关于在原代培养中分离副结核分枝杆菌的观察
J Pathol Bacteriol. 1950 Oct;62(4):647-50. doi: 10.1002/path.1700620414.
3
VACCINATION AGAINST JOHNE'S DISEASE IN CATTLE EXPOSED TO EXPERIMENTAL INFECTION.对暴露于实验性感染的牛进行副结核菌素病疫苗接种。
Br Vet J. 1965 Jul;121:289-318. doi: 10.1016/s0007-1935(17)41102-x.
4
MEDIA FOR MYCOBACTERIUM JOHNEI.副结核分枝杆菌培养基
Natl Inst Anim Health Q (Tokyo). 1965;5:105-6.
5
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Am J Vet Res. 1964 Jul;25:1290-4.
6
Modifications of Dubos's media for the cultivation of Mycobacterium johnei.
J Pathol Bacteriol. 1953 Oct;66(2):375-81. doi: 10.1002/path.1700660206.
7
Methods for selective isolation of mycobacteria from the environment.
Can J Microbiol. 1981 Jan;27(1):1-7. doi: 10.1139/m81-001.
8
Isolation of mycobacteria from undecontaminated specimens with selective 7H10 medium.使用选择性7H10培养基从未经污染的标本中分离分枝杆菌。
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9
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