Fang Zongxi Center for Marine Evo-Devo & MOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao, China.
Laboratory for Marine Biology and Biotechnology, Laoshan Laboratory, Qingdao, China.
Commun Biol. 2024 Jul 7;7(1):827. doi: 10.1038/s42003-024-06509-7.
The "hologenome" concept is an increasingly popular way of thinking about microbiome-host for marine organisms. However, it is challenging to track hologenome dynamics because of the large amount of material, with tracking itself usually resulting in damage or death of the research object. Here we show the simple and efficient holo-2bRAD approach for the tracking of hologenome dynamics in marine invertebrates (i.e., scallop and shrimp) from one holo-2bRAD library. The stable performance of our approach was shown with high genotyping accuracy of 99.91% and a high correlation of r > 0.99 for the species-level profiling of microorganisms. To explore the host-microbe association underlying mass mortality events of bivalve larvae, core microbial species changed with the stages were found, and two potentially associated host SNPs were identified. Overall, our research provides a powerful tool with various advantages (e.g., cost-effective, simple, and applicable for challenging samples) in genetic, ecological, and evolutionary studies.
“全基因组”概念是一种越来越流行的思考海洋生物微生物组-宿主的方式。然而，由于材料数量庞大，跟踪全基因组动态具有挑战性，通常会导致研究对象的损伤或死亡。在这里，我们展示了一种简单而高效的 holo-2bRAD 方法，用于从一个 holo-2bRAD 文库中跟踪海洋无脊椎动物（即扇贝和虾）的全基因组动态。我们的方法具有稳定的性能，其基因型准确率高达 99.91%，微生物物种水平分析的相关性 r>0.99。为了探索双壳类幼虫大规模死亡事件背后的宿主-微生物关联，我们发现了与阶段变化相关的核心微生物物种，并鉴定了两个可能相关的宿主 SNPs。总的来说，我们的研究为遗传、生态和进化研究提供了一种具有各种优势（例如，具有成本效益、简单和适用于具有挑战性的样本）的强大工具。
