Andrianov V M, Amerkhanova M B, Vinetskiĭ Iu P
Genetika. 1979 Nov;15(11):1918-24.
Previously a method of selection of colicine-defective recombinant plasmids by mitomycin C was described. A series of recombinant plasmids (CPS) with various EcoRI-fragments of pea chloroplast DNA has been obtained. This paper describes some properties of cloned fragments replicated in Escherichia coli. The alkali stability of recombinant plasmid DNAs has been demonstrated, indicating the absence of ribonucleotides in their structure. Heterogeneity of chloroplast DNA in nucleotide composition was demonstrated using ultracentrifugation analysis of CPS-plasmid DNAs in CsCl-actinomycin D density gradient. Pea chloroplast rDNA was cloned in recombinant plasmids.
此前已描述了一种通过丝裂霉素C筛选大肠杆菌素缺陷型重组质粒的方法。已获得一系列含有豌豆叶绿体DNA不同EcoRI片段的重组质粒（CPS）。本文描述了在大肠杆菌中复制的克隆片段的一些特性。已证明重组质粒DNA的碱稳定性，表明其结构中不存在核糖核苷酸。通过在CsCl-放线菌素D密度梯度中对CPS-质粒DNA进行超速离心分析，证明了叶绿体DNA在核苷酸组成上的异质性。豌豆叶绿体rDNA被克隆到重组质粒中。
