纤细裸藻叶绿体rRNA编码基因区域的分子克隆
Molecular cloning of the gene region coding for the chloroplast rRNA of Euglena gracilis.
作者信息
Knopf U C, Stutz E
出版信息
Mol Gen Genet. 1978 Jul 6;163(1):1-6. doi: 10.1007/BF00268957.
PMID:98702
Abstract
E. gracilis chloroplast DNA Bam fragments E and D, coding for rRNA were cloned separately using the plasmid pBR 322 as vector and E. coli as host. The newly constructed recombinant plasmids EgcKS 8 and EgcKS 11 (containing the Bam HI fragments E and D respectively) were analysed and characterized by gel electrophoresis, electronmicroscopy and analytical ultracentrifugation.
摘要
纤细裸藻叶绿体DNA的Bam片段E和D编码rRNA，分别以质粒pBR 322为载体、大肠杆菌为宿主进行克隆。对新构建的重组质粒EgcKS 8和EgcKS 11（分别含有Bam HI片段E和D）进行凝胶电泳、电子显微镜检查和分析超速离心分析及表征。
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