Wu Jiacheng, Shen Hexiao, Lv Yongling, He Jing, Xie Xiaotian, Xu Zhiyue, Yang Pengcheng, Qian Wei, Bai Tao, Hou Xiaohua
Division of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
School of Life Science, Hubei University, Wuhan, Hubei, China.
Front Microbiol. 2024 Jun 21;15:1412991. doi: 10.3389/fmicb.2024.1412991. eCollection 2024.
Age and gender have been recognized as two pivotal covariates affecting the composition of the gut microbiota. However, their mediated variations in microbiota seem to be inconsistent across different countries and races. In this study, 613 individuals, whom we referred to as the "healthy" population, were selected from 1,018 volunteers through rigorous selection using 16S rRNA sequencing. Three enterotypes were identified, namely, , mixture ( and ), and . Moreover, 11 covariates that explain the differences in microbiota were determined, with age being the predominant factor. Furthermore, age-related differences in alpha diversity, beta diversity, and core genera were observed in our cohort. Remarkably, after adjusting for 10 covariates other than age, abundant genera that differed between age groups were demonstrated. In contrast, minimal differences in alpha diversity, beta diversity, and differentially abundant genera were observed between male and female individuals. Furthermore, we also demonstrated the age trajectories of several well-known beneficial genera, lipopolysaccharide (LPS)-producing genera, and short-chain fatty acids (SCFAs)-producing genera. Overall, our study further elucidated the effects mediated by age and gender on microbiota differences, which are of significant importance for a comprehensive understanding of the gut microbiome spectrum in healthy individuals.
年龄和性别已被公认为影响肠道微生物群组成的两个关键协变量。然而，它们在微生物群中的介导变化在不同国家和种族之间似乎并不一致。在本研究中，通过16S rRNA测序进行严格筛选，从1018名志愿者中选出613名个体，我们将其称为“健康”人群。确定了三种肠型，即，混合型（和），以及。此外，确定了11个解释微生物群差异的协变量，其中年龄是主要因素。此外，在我们的队列中观察到了与年龄相关的α多样性、β多样性和核心属的差异。值得注意的是，在调整了除年龄以外的10个协变量后，证明了年龄组之间存在差异的丰富属。相比之下，在男性和女性个体之间，观察到α多样性、β多样性和差异丰富属的差异最小。此外，我们还展示了几种著名的有益属、产生脂多糖（LPS）的属和产生短链脂肪酸（SCFAs）的属的年龄轨迹。总体而言，我们的研究进一步阐明了年龄和性别对微生物群差异的介导作用，这对于全面了解健康个体的肠道微生物组谱具有重要意义。
