Neonatal Intensive Care Unit, Perth Children's Hospital and King Edward Memorial Hospital for Women, Perth, WA, Australia.
Centre for Neonatal Research and Education, University of Western Australia, Perth, WA, Australia.
Pediatr Res. 2020 Dec;88(6):878-886. doi: 10.1038/s41390-020-0824-7. Epub 2020 Mar 16.
There is limited information on gut microbiota of neonates with congenital gastrointestinal surgical conditions (CGISCs) available.
This study compared stool microbiota and short-chain fatty acids (SCFAs) of 37 term infants with CGISCs with 36 term healthy infants (HIs). Two stool samples were collected from each infant: as soon as possible after birth (week 1) and 10-14 days of life (week 2).
Bacterial richness and alpha diversity were comparable between CGISCs and HIs at week 1 and week 2 (all p > 0.05). Beta diversity analysis revealed that at week 1, CGISCs had similar community structures to HIs (p = 0.415). However, by week 2, community structures of CGISCs were significantly different from HIs (p = 0.003). At week 1, there were no significant differences in the relative abundances of genera Bifidobacterium and Bacteroides between CGISCs and HIs. At week 2, the relative abundance of Bifidobacterium was significantly lower in CGISCs (mean percentage 7.21 ± 13.49 vs. 28.96 ± 19.6; p = 0.002). Bacteroides were also less abundant in the CGISC group (mean percentage 0.12 ± 0.49 vs. 6.59 ± 8.62; p = 0.039). Relative abundance of genera Pseudomonas and Escherichia-Shigella were higher in CGISCs. At week 2, stool concentrations of all SCFAs were lower in CGISCs (all p < 0.001).
During hospitalization, neonates with CGISCs develop gut dysbiosis and deficiency of SCFAs.
During hospitalisation, neonates with congenital gastrointestinal surgical conditions develop gut dysbiosis with deficiency of Bifidobacteria and Bacteroides and increased abundance of Escherichia-Shigella and Pseudomonas. They also have low levels of short chain fatty acids in their stools compared to healthy infants. This is the first study evaluating the gut microbiota using 16S ribosomal RNA sequencing methods and stool short chain fatty acids in neonates with congenital gastrointestinal surgical conditions and comparing them to healthy infants. The findings of this study will pave the way for randomised trials of bifidobacterial supplementation in neonates with congenital gastrointestinal surgical conditions.
关于先天性胃肠道外科疾病（CGISCs）新生儿的肠道微生物组的信息有限。
本研究比较了 37 例 CGISCs 足月婴儿和 36 例足月健康婴儿（HI）的粪便微生物组和短链脂肪酸（SCFAs）。从每个婴儿收集两份粪便样本：出生后尽快（第 1 周）和第 10-14 天（第 2 周）。
在第 1 周和第 2 周，CGISCs 和 HI 的细菌丰富度和 alpha 多样性无差异（均 p>0.05）。β多样性分析显示，第 1 周 CGISCs 的群落结构与 HI 相似（p=0.415）。然而，第 2 周时，CGISCs 的群落结构与 HI 有显著差异（p=0.003）。第 1 周时，CGISCs 和 HI 之间双歧杆菌属和拟杆菌属的相对丰度无显著差异。第 2 周时，CGISCs 中双歧杆菌属的相对丰度明显较低（平均百分比 7.21±13.49 比 28.96±19.6；p=0.002）。拟杆菌属在 CGISC 组也较少（平均百分比 0.12±0.49 比 6.59±8.62；p=0.039）。CGISCs 中假单胞菌属和大肠埃希氏菌-志贺菌属的相对丰度较高。第 2 周时，CGISCs 组的所有 SCFA 浓度均较低（均 p<0.001）。
住院期间，CGISCs 新生儿出现肠道菌群失调和 SCFA 缺乏。
住院期间，CGISCs 新生儿出现双歧杆菌属和拟杆菌属减少、大肠埃希氏菌-志贺菌属和假单胞菌属增加的肠道菌群失调，且粪便中短链脂肪酸水平较低。与健康婴儿相比，这是第一项使用 16S 核糖体 RNA 测序方法评估 CGISCs 新生儿肠道微生物组并将其与健康婴儿进行比较的研究。本研究的发现将为 CGISCs 新生儿双歧杆菌补充的随机试验铺平道路。
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