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大豆（）和玉米（）混合品系的同时遗传转化和基因组编辑。

Simultaneous genetic transformation and genome editing of mixed lines in soybean () and maize ().

作者信息

Valentine Michelle, Butruille David, Achard Frederic, Beach Steven, Brower-Toland Brent, Cargill Edward, Hassebrock Megan, Rinehart Jennifer, Ream Thomas, Chen Yurong

机构信息

Bayer Crop Science, 700 Chesterfield Parkway W, Chesterfield, MO 63017 USA.

出版信息

aBIOTECH. 2024 Jun 18;5(2):169-183. doi: 10.1007/s42994-024-00173-5. eCollection 2024 Jun.

DOI:10.1007/s42994-024-00173-5
PMID:38974857
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11224177/
Abstract

UNLABELLED

Robust genome editing technologies are becoming part of the crop breeding toolbox. Currently, genome editing is usually conducted either at a single locus, or multiple loci, in a variety at one time. Massively parallel genomics platforms, multifaceted genome editing capabilities, and flexible transformation systems enable targeted variation at nearly any locus, across the spectrum of genotypes within a species. We demonstrate here the simultaneous transformation and editing of many genotypes, by targeting mixed seed embryo explants with genome editing machinery, followed by re-identification through genotyping after plant regeneration. Transformation and Editing of Mixed Lines (TREDMIL) produced transformed individuals representing 101 of 104 (97%) mixed elite genotypes in soybean; and 22 of 40 (55%) and 9 of 36 (25%) mixed maize female and male elite inbred genotypes, respectively. Characterization of edited genotypes for the regenerated individuals identified over 800 distinct edits at the Determinate1 () locus in samples from 101 soybean genotypes and 95 distinct Brown midrib3 () edits in samples from 17 maize genotypes. These results illustrate how TREDMIL can help accelerate the development and deployment of customized crop varieties for future precision breeding.

SUPPLEMENTARY INFORMATION

The online version contains supplementary material available at 10.1007/s42994-024-00173-5.

摘要

未标注

强大的基因组编辑技术正成为作物育种工具箱的一部分。目前，基因组编辑通常在一个品种的单个位点或多个位点同时进行。大规模平行基因组学平台、多方面的基因组编辑能力和灵活的转化系统能够在一个物种的各种基因型范围内，对几乎任何位点进行靶向变异。我们在此展示了通过用基因组编辑工具靶向混合种子胚外植体，然后在植物再生后通过基因分型重新鉴定，对多种基因型进行同时转化和编辑。混合品系的转化与编辑（TREDMIL）在大豆中产生了代表104个混合优良基因型中101个（97%）的转化个体；在玉米中，分别产生了代表40个混合优良雌性自交基因型中22个（55%）和36个混合优良雄性自交基因型中9个（25%）的转化个体。对再生个体的编辑基因型进行表征，在来自101个大豆基因型的样本中，在Determinate1（）位点鉴定出800多个不同的编辑，在来自17个玉米基因型的样本中，在Brown midrib3（）位点鉴定出95个不同的编辑。这些结果说明了TREDMIL如何有助于加速未来精准育种中定制作物品种的开发和应用。

补充信息

在线版本包含可在10.1007/s42994-024-00173-5获取的补充材料。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b09d/11224177/1821a5209caf/42994_2024_173_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b09d/11224177/5a203c84fcaf/42994_2024_173_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b09d/11224177/b73d4c5515a3/42994_2024_173_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b09d/11224177/9aa57e01abbb/42994_2024_173_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b09d/11224177/896853e36012/42994_2024_173_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b09d/11224177/ef740cd75fd5/42994_2024_173_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b09d/11224177/1821a5209caf/42994_2024_173_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b09d/11224177/5a203c84fcaf/42994_2024_173_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b09d/11224177/b73d4c5515a3/42994_2024_173_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b09d/11224177/9aa57e01abbb/42994_2024_173_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b09d/11224177/896853e36012/42994_2024_173_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b09d/11224177/ef740cd75fd5/42994_2024_173_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b09d/11224177/1821a5209caf/42994_2024_173_Fig6_HTML.jpg

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