Cytokine-like-Vago-mediated antiviral response in via IKK-NF-κB signaling pathway.

Interferon (IFN) system is the primary mechanism of innate antiviral defense in immune response. To date, limited studies of IFN system were conducted in crustaceans. Previous report in demonstrated the interconnection of cytokine-like molecule Vago and inhibitor of kappa B kinase-nuclear factor κB (IKK-NF-κB) cascade against white spot syndrome virus (WSSV). This study further identified five different isoforms. Upon immune stimulation, expressed against shrimp pathogens. , and highly responded to WSSV, whereas, and RNAi exhibited a rapid mortality with elevated WSSV replication. Suppression of and negatively affected proPO system, genes in signal transduction, and AMPs. WSSV infection additionally induced Vaog4 granule accumulation and cellular translocation to the area of cell membrane. More importantly, and promoters were stimulated by IKK overexpression; meanwhile, they further activated and promoter activities. These results suggested the possible roles of the cytokine-like via IKK-NF-κB cascade against WSSV infection.