Lucas R M, Subramoniam A, Aleo J J
J Periodontol. 1985 Sep;56(9):553-7. doi: 10.1902/jop.1985.56.9.553.
The intracellular localization in 3T6 fibroblasts of Escherichia coli lipopolysaccharide (LPS) using the rapid avidin-biotin-immunoperoxidase technique at the electron microscopic level was studied. The role of bacterial endotoxin in the etiology of periodontal disease has been well documented previously. The purpose of the present study was to localize LPS within the cell, thereby determining which organelles concentrate the material and relate this to the cytologic pathophysiology. An increased concentration of LPS was found in the cell nuclei and, specifically, in association with nuclear chromatin and nucleoli. The concentration of LPS in the nucleus was directly related to the time of incubation, with some product appearing in that site within 2 minutes. There was no specific localization of endotoxin in mitochondria, lysosomes, Golgi, endoplasmic reticulum or ribosomes. These results imply that bacterial endotoxin may have a direct effect on nuclear components of fibroblasts. The relationship of these results to the etiologic mechanisms of periodontal disease is discussed.
利用快速抗生物素蛋白-生物素-免疫过氧化物酶技术，在电子显微镜水平上研究了大肠杆菌脂多糖（LPS）在3T6成纤维细胞内的定位。细菌内毒素在牙周病病因学中的作用此前已有充分记载。本研究的目的是在细胞内定位LPS，从而确定哪些细胞器浓缩了该物质，并将其与细胞病理生理学联系起来。结果发现细胞核内LPS浓度增加，特别是与核染色质和核仁相关。细胞核内LPS的浓度与孵育时间直接相关，2分钟内该部位就出现了一些产物。内毒素在线粒体、溶酶体、高尔基体、内质网或核糖体中没有特异性定位。这些结果表明细菌内毒素可能对成纤维细胞的核成分有直接影响。讨论了这些结果与牙周病病因机制的关系。
