DeRenzis F A, Chen S Y
J Periodontol. 1983 Feb;54(2):86-90. doi: 10.1902/jop.1983.54.2.86.
Our current concept of periodontal disease is that some mediators found in the gingival sulcus are able to penetrate the epithelial barrier and produce tissue destruction in the periodontium. Endotoxin, one such mediator, recently has been the subject of considerable study. The purpose of this investigation was to substantiate the role of endotoxin in the pathogenesis of periodontal disease by ascertaining its action on cellular and subcellular components. In order to accomplish this, cultured human gingival fibroblasts were exposed to media containing either 300 micrograms/ml or 500 micrograms/ml of endotoxin and untreated medium for a period of 24 hours. Cells were harvested and prepared for electron microscopy by conventional methods. Electron microscopic examination revealed alterations in mitochondria, Golgi apparatus, lysosomal-like bodies, cytofilaments and plasma membrane. Relationship of these alterations to pathologic processes is discussed.
我们目前对于牙周病的认识是，在龈沟中发现的某些介质能够穿透上皮屏障并在牙周组织中造成组织破坏。内毒素就是这样一种介质，最近它已成为大量研究的对象。本研究的目的是通过确定内毒素对细胞和亚细胞成分的作用，来证实其在牙周病发病机制中的作用。为了实现这一目标，将培养的人牙龈成纤维细胞暴露于含有300微克/毫升或500微克/毫升内毒素的培养基以及未处理的培养基中24小时。通过常规方法收获细胞并制备用于电子显微镜检查。电子显微镜检查发现线粒体、高尔基体、溶酶体样小体、细胞丝和质膜发生了改变。讨论了这些改变与病理过程的关系。
