Kurki P, Virtanen I, Lehto V P, Helve T
J Rheumatol. 1985 Apr;12(2):253-6.
We describe a new type of human antinuclear antibody (ANA) reacting with the nuclear matrix by indirect immunofluorescence technique. Cultured cells of human and animal origin were used as substrate. Before assay, cells were extracted with buffers to remove other components of the nuclei leaving the matrix intact. Two types of immunofluorescence staining patterns were seen: homogenous and speckled. When human embryonic fibroblasts were used as targets, sera from patients with systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA) contained significantly more nuclear matrix antibodies of the homogenous type than the control group. These antibodies were often, especially in SLE, species cross-reacting. (When PTK-2 cells were used as substrate the difference between SLE and RA was also significant [60 vs 8%]). Antibodies with speckled staining pattern were seen only in the patient sera. Their highest incidence (35%) was found in sera from patients with the primary sicca syndrome. The nuclear matrix may be a major target for ANA in rheumatic diseases.
我们描述了一种通过间接免疫荧光技术与核基质发生反应的新型人类抗核抗体（ANA）。使用人和动物来源的培养细胞作为底物。在检测前，用缓冲液提取细胞以去除细胞核的其他成分，使核基质保持完整。观察到两种免疫荧光染色模式：均匀型和斑点型。当使用人胚胎成纤维细胞作为靶标时，系统性红斑狼疮（SLE）和类风湿性关节炎（RA）患者的血清中所含均匀型核基质抗体明显多于对照组。这些抗体通常具有种属交叉反应性，尤其是在SLE患者中。（当使用PTK - 2细胞作为底物时，SLE和RA之间的差异也很显著[60%对8%]）。仅在患者血清中观察到斑点染色模式的抗体。在原发性干燥综合征患者的血清中发现其发生率最高（35%）。核基质可能是风湿性疾病中ANA的主要靶标。
