The Niels Bohr Institute, University of Copenhagen, Copenhagen, Denmark.
PLoS Comput Biol. 2024 Jul 8;20(7):e1012242. doi: 10.1371/journal.pcbi.1012242. eCollection 2024 Jul.
T-even bacteriophages are known to employ lysis inhibition (LIN), where the lysis of an infected host is delayed in response to secondary adsorptions. Upon the eventual burst of the host, significantly more phage progenies are released. Here, we analysed the competitive advantage of LIN using a mathematical model. In batch culture, LIN provides a bigger phage yield at the end of the growth where all the hosts are infected due to an exceeding number of phage particles and, in addition, gives a competitive advantage against LIN mutants with rapid lysis by letting them adsorb to already infected hosts in the LIN state. By simulating plaque formation in a spatially structured environment, we show that, while LIN phages will produce a smaller zone of clearance, the area over which the phages spread is actually comparable to those without LIN. The analysis suggests that LIN induced by secondary adsorption is favourable in terms of competition, both in spatially homogeneous and inhomogeneous environments.
T 偶数噬菌体被认为采用裂解抑制（LIN），即感染宿主的裂解会因二次吸附而延迟。最终宿主破裂时，会释放出更多的噬菌体后代。在这里，我们使用数学模型分析了 LIN 的竞争优势。在分批培养中，由于噬菌体颗粒数量过多，所有宿主都会被感染，因此在生长结束时 LIN 会提供更大的噬菌体产量，并且通过让它们吸附到已经感染的 LIN 状态的宿主上，LIN 还对快速裂解的 LIN 突变体具有竞争优势。通过在空间结构环境中模拟菌斑形成，我们表明，虽然 LIN 噬菌体产生的清除区域较小，但噬菌体传播的区域实际上与没有 LIN 的区域相当。分析表明，在空间均匀和不均匀的环境中，由二次吸附引起的 LIN 在竞争中是有利的。
