College of Optoelectronics Technology, Shuangliu Industry College, Information Materials and Device Applications Key Laboratory of Sichuan Provincial Universities, Chengdu University of Information Technology, Chengdu 610225, China.
Institute of Hybrid Materials, College of Materials Science and Engineering, Qingdao University, Qingdao, 266071, P. R. China.
Analyst. 2024 Jul 22;149(15):3961-3970. doi: 10.1039/d4an00689e.
Manganese dioxide (MnO) nanosheets possess unique physical and chemical properties, making them widely applicable in various fields, such as chemistry and biomedicine. Although MnO nanosheets are produced using bottom-up wet chemistry synthesis methods, their scale is below the gram level and requires a long processing time, restricting their effective scale-up from laboratory to market. We report a facile, green and scalable synthesis of MnO nanosheets by mixing Shiranui mandarin orange juice and KMnO for 30 minutes. We produced more than one gram (1.095) of MnO nanosheets with a 0.65 nm mean thickness and a 50 nm mean lateral size. Furthermore, we established a visual colorimetric biosensing strategy based on MnO nanosheets for the assay of glutathione (GSH) and cardiac troponin I (cTnI), offering high sensitivity and feasibility in clinical samples. For GSH, the limit of detection was 0.08 nM, and for cTnI, it was 0.70 pg mL. Meanwhile, the strategy can be used for real-time analysis by applying a smartphone-enabled biosensing strategy, which can provide point-of-care testing in remote areas.
二氧化锰（MnO）纳米片具有独特的物理和化学性质，因此在化学和生物医药等各个领域都有广泛的应用。虽然 MnO 纳米片可以采用自下而上的湿化学合成方法制备，但它们的规模在克以下，且需要较长的处理时间，这限制了其从实验室规模扩大到市场规模的应用。我们报告了一种简便、绿色、可扩展的合成 MnO 纳米片的方法，即将蜜柑汁和 KMnO 混合并反应 30 分钟。我们成功制备了超过 1 克（1.095 克）的 MnO 纳米片，其平均厚度为 0.65nm，平均横向尺寸为 50nm。此外，我们还建立了一种基于 MnO 纳米片的可视化比色生物传感策略，用于测定谷胱甘肽（GSH）和心肌肌钙蛋白 I（cTnI），该策略在临床样本中具有高灵敏度和可行性。对于 GSH，检测限为 0.08 nM，对于 cTnI，检测限为 0.70 pg/mL。同时，该策略还可以通过应用智能手机辅助的生物传感策略进行实时分析，从而可以在偏远地区提供即时检测。
