School of Materials Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, PR China.
School of National Defence Science & Technology, Southwest University of Science and Technology, Mianyang 621010, PR China.
Biosens Bioelectron. 2017 May 15;91:89-94. doi: 10.1016/j.bios.2016.12.028. Epub 2016 Dec 13.
Here we report a unique visual colorimetric sensor array for discrimination of antioxidants in serum based on MnO nanosheets-3,3',5,5'-tetramethylbenzidine (TMB) multicolor chromogenic system. The absorbance values of the system at 370, 450, and 650nm provide three cross-reactive sensing elements. The presence of antioxidant will inhibit the reaction between TMB and MnO nanosheets due to the presence of the competitive reaction of MnO nanosheets and antioxidants. Different antioxidants containing uric acid, glutathione, ascorbic acid, cysteine, and melatonin have distinct reducing ability, producing a differential inhibition of MnO nanosheets-TMB system, and therefore generating distinct colorimetric response patterns at 370, 450, and 650nm. The obtained patterns for each antioxidant at a concentration of 20μM were successfully discriminated using principal component analysis both in buffer and when spiked into fetal bovine serum (FBS). The identification accuracy of 45 unknown samples was found to be 100%. Remarkably, this sensor assay can visually discriminate antioxidants in diluted FBS with the naked eye.
在这里，我们报告了一种基于 MnO 纳米片-3,3',5,5'-四甲基联苯胺（TMB）多色显色体系的用于区分血清中抗氧化剂的独特可视化比色传感器阵列。该体系在 370、450 和 650nm 处的吸光度值提供了三个交叉反应的传感元件。由于 MnO 纳米片和抗氧化剂之间存在竞争性反应，抗氧化剂的存在会抑制 TMB 和 MnO 纳米片之间的反应。具有不同还原能力的不同抗氧化剂，如尿酸、谷胱甘肽、抗坏血酸、半胱氨酸和褪黑素，对 MnO 纳米片-TMB 体系产生不同的抑制作用，因此在 370、450 和 650nm 处产生不同的比色响应模式。在缓冲液和添加到胎牛血清（FBS）中时，使用主成分分析成功区分了每种抗氧化剂在 20μM 浓度下的获得的模式。发现对 45 个未知样品的识别准确率为 100%。值得注意的是，这种传感器检测法可以用肉眼可视化区分稀释 FBS 中的抗氧化剂。
